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Products:Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Overview
Product name
Anti-SAPS3 antibody
See all SAPS3 products (2) ...
Description
Rabbit polyclonal to SAPS3
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from a region between residue 743 and the C-terminus (residue 793) of human SAPS3,( NP_060782.2).
Positive control
Whole cell lysate from Hela cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72034 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 120 kDa (predicted molecular weight: 98 kDa).
IP: Use at 2-5 µg/mg of lysate.
Target
Function
Regulatory subunit of protein phospatase 6 (PP6). May function as a scaffolding PP6 subunit. May have an important role in maintaining immune self-tolerance.
Tissue specificity
Expressed in skeletal muscle, placenta, heart, pancreas, testis, brain, lung, liver, kidney, spleen, thymus, prostate, small intestine, colon and leukocytes.
Sequence similarities
Belongs to the SAPS family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ5H9R7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C11orf23 antibody
- Chromosome 11 open reading frame 23 antibody
- KIAA1558 antibody
see all
Database links
- Entrez Gene: 55291 Human
- Omim: 610879 Human
- SwissProt: Q5H9R7 Human
- Unigene: 503022 Human
Anti-SAPS3 antibody images:
Western blot - SAPS3 antibody (ab72034)
All lanes : Anti-SAPS3 antibody (ab72034) at 0.02 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Predicted band size : 98 kDa
Observed band size : 120 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - SAPS3 antibody (ab72034)
Immunoprecipitation/ Western Blot of SAPS3.
Lane 1: For IP: ab72034 at 3µg/mg whole cell lysate, with ab72034 at 0.04µg/ml for subsequent WB.
Lane 2: Control IgG.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
References for Anti-SAPS3 antibody (ab72034)
ab72034 has not yet been referenced specifically in any publications.
Publishing research using ab72034? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"