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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-SAR1 antibody
See all SAR1 products (3) ...
Description
Mouse monoclonal to SAR1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human) (AAH03658) with a 26 kDa tag.
Positive control
Jurkat cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 22 kDa.
ELISA: Use at an assay dependent dilution.
Target
Function
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Sequence similarities
Belongs to the small GTPase superfamily. SAR1 family.
Cellular localization
Endoplasmic reticulum. Golgi apparatus.
Target information above from: UniProt accessionQ9NR31
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COPII associated small GTPase antibody
- COPII-associated small GTPase antibody
- GTP binding protein SAR1a antibody
see all
Database links
- Entrez Gene: 56681 Human
- Omim: 607691 Human
- SwissProt: Q9NR31 Human
- Unigene: 499960 Human
- Unigene: 594910 Human
Anti-SAR1 antibody images:
Western blot - SAR1 antibody (ab77029)
Anti-SAR1 antibody (ab77029) at 5 µg/ml + recombinant full length human SAR1 protein with a tag (immunogen) at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
Note: Molecular weight of the tag alone is 26 kDa.
Western blot - SAR1 antibody (ab77029)
Anti-SAR1 antibody (ab77029) at 5 µg/ml + Jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
References for Anti-SAR1 antibody (ab77029)
ab77029 has not yet been referenced specifically in any publications.
Publishing research using ab77029? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"