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Western blot - SAR1 antibody (ab77029)
(enlarge)
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Western blot - SAR1 antibody (ab77029)
(enlarge)
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Product Name
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SAR1 antibody
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See all SAR1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to SAR1
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Immunogen
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Recombinant full length protein (Human) (AAH03658) with a 26 kDa tag.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Jurkat cell lysate.
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Cellular localization
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Endoplasmic reticulum and Golgi Apparatus
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Research areas
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Signal Transduction >> Protein Trafficking >> Organelle Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Relevance
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SAR1 is involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). It belongs to the small GTPase superfamily. SAR1 family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 1X PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SAR1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SAR1 antibody images, references, products related to ab77029 and other tools.
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SAR1 antibody images:
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 Western blot - SAR1 antibody (ab77029)
SAR1 antibody (ab77029) at 5 µg/ml + recombinant full length human SAR1 protein with a tag (immunogen) at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
Note: Molecular weight of the tag alone is 26 kDa.
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Western blot - SAR1 antibody (ab77029)
SAR1 antibody (ab77029) at 5 µg/ml + Jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 20 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to SAR1
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SAR1 antibody - more information
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Customer reviews (feedback) regarding SAR1 antibody |
Customer FAQs regarding SAR1 antibody |
Protocols for SAR1 antibody |
Price and availability of products related to SAR1 antibody |
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SAR1 antibody - related products:
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SAR1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SAR1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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