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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-SAR1 antibody
See all SAR1 products (3) ...
Description
Goat polyclonal to SAR1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human, Chinese Hamster, Orangutan
Immunogen
Synthetic peptide: HSRLVESKVELNA (Human) from the internal region of the protein sequence according to NP_064535.1.
HSRLVESKVE LNA
Positive control
NIH3T3 cell lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Western blot - SAR1 antibody (ab77473)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Sequence similarities
Belongs to the small GTPase superfamily. SAR1 family.
Cellular localization
Endoplasmic reticulum. Golgi apparatus.
Target information above from: UniProt accessionQ9NR31
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- COPII associated small GTPase antibody
- COPII-associated small GTPase antibody
- GTP binding protein SAR1a antibody
see all
Database links
- Entrez Gene: 56681 Human
- Entrez Gene: 20224 Mouse
- Entrez Gene: 100173852 Orangutan
- Entrez Gene: 361842 Rat
- Omim: 607691 Human
- SwissProt: Q9NR31 Human
- SwissProt: P36536 Mouse
- SwissProt: Q5R548 Orangutan
see all
Anti-SAR1 antibody images:
Western blot - SAR1 antibody (ab77473)
Anti-SAR1 antibody (ab77473) at 0.1 µg/ml + NIH3T3 cell lysates in RIPA buffer at 35 µg
Predicted band size : 25 kDa
Observed band size : 23 kDa (why is the actual band size different from the predicted?)
References for Anti-SAR1 antibody (ab77473)
ab77473 has not yet been referenced specifically in any publications.
Publishing research using ab77473? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"