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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
SAR1 protein (T7 Tag)
See all SAR1 products (2) ...
Protein description
Recombinant full length protein: Human SAR1 fused to T7 tag at N-terminus.
Expression host
E. coli
Properties
Purity
>95% by SDS-PAGE
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.002% Sodium Azide
Constituents: 0.1% Triton-X-100, 10mM Tris, 10mM DTT, pH 8
Concentration
Concentration information loading...
Research areas
Signal Transduction >> Protein Trafficking >> Organelle Proteins
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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SDS-PAGE - SAR1 protein (ab40534)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab40534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
SDS-PAGE
IP
Application notes
IP: Use at a concentration of 1.2 µg/ml (PMID 19181664).
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
Sequence similarities
Belongs to the small GTPase superfamily. SAR1 family.
Cellular localization
Endoplasmic reticulum. Golgi apparatus.
Target information above from: UniProt accessionQ9NR31
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
SAR1 protein (T7 Tag) images:
SDS-PAGE - SAR1 protein (ab40534)
SDS-PAGE Analysis of SAR1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
References for SAR1 protein (T7 Tag) (ab40534)
This product has been referenced in:
- Kizuka Yet al. Distinct Transport and Intracellular Activities of Two GlcAT-P Isoforms. J Biol Chem 284:9247-56 (2009). IP.Read more (PubMed: 19181664) »
See 1 publication for this product
Publishing research using ab40534? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"