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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-SAR1B antibody
See all SAR1B products (2) ...
Description
Rabbit polyclonal to SAR1B
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide derived from internal sequence of human SAR1B.
Positive control
Human placenta tissue. Extracts from LOVO and RAW264.7 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> ER Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
ELISA
ELISA: 1/20000ELISA: 1/20000
IHC-P: 1/50 - 1/100.
Target
Relevance
SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region.
Alternative names
- CMRD antibody
- GTBPB antibody
- GTP binding protein SAR1b antibody
see all
Database links
- Entrez Gene: 51128 Human
- Entrez Gene: 66397 Mouse
- Entrez Gene: 287276 Rat
- Omim: 607690 Human
- SwissProt: Q9Y6B6 Human
- SwissProt: Q9CQC9 Mouse
- SwissProt: Q5HZY2 Rat
Anti-SAR1B antibody images:
Western blot - SAR1B antibody (ab74046)
All lanes : Anti-SAR1B antibody (ab74046) at 1/500 dilution
Lane 1 : Extracts from LOVO cells
Lane 2 : Extracts from RAW264.7 cells
Lane 3 : Extracts from LOVO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 22 kDa
Observed band size : 22 kDa
Additional bands at : 21 kDa,27 kDa,35 kDa,40 kDa,55 kDa,87 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SAR1B antibody (ab74046)
Immunohistochemistry analysis of paraffin-embedded human placenta tissue using ab74046, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
References for Anti-SAR1B antibody (ab74046)
ab74046 has not yet been referenced specifically in any publications.
Publishing research using ab74046? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"