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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Overview
Product name
Anti-SAR1B antibody
See all SAR1B products (2) ...
Description
Goat polyclonal to SAR1B
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide: DHERLLESKEELD, corresponding to internal sequence amino acids 104-116 of Human SAR1B (NP_001028675.1; NP_057187.1)
DHERLLESKE ELD
Positive control
Mouse and Rat Liver lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> ER Proteins
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
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Western blot - SAR1B antibody (ab77279)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: antibody detection limit dilution: 1/8000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Associated with the endoplasmic reticulum and Golgi stacks, in particular in the juxta-nuclear Golgi region.
Alternative names
- CMRD antibody
- GTBPB antibody
- GTP binding protein SAR1b antibody
see all
Database links
- Entrez Gene: 51128 Human
- Entrez Gene: 66397 Mouse
- Entrez Gene: 287276 Rat
- Omim: 607690 Human
- SwissProt: Q9Y6B6 Human
- SwissProt: Q9CQC9 Mouse
- SwissProt: Q5HZY2 Rat
Anti-SAR1B antibody images:
Western blot - SAR1B antibody (ab77279)
Anti-SAR1B antibody (ab77279) at 0.03 µg/ml + Mouse Liver lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
References for Anti-SAR1B antibody (ab77279)
ab77279 has not yet been referenced specifically in any publications.
Publishing research using ab77279? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"