|
|
|
|
Western blot - SART1 antibody (ab22820)
(enlarge)
|
|
|
|
|
Product Name
|
SART1 antibody
|
| |
See all SART1 antibodies (2)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Goat polyclonal to SART1
|
|
Immunogen
|
Synthetic peptide: GSSKKHRGEKEAA, corresponding to N terminal amino acids 2-14 of Human SART1.
BLAST 'GSSKKHRGEKEAA' with  or 
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Ms, Rat
(Due to sequence homology)
|
|
Specificity
|
ab22820 recognises both the human SART1(259) and SART1(800) proteins.
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: 1/64000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 90 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa cell lysate
|
|
Cellular localization
|
Cytoplasmic and Nuclear
|
|
Research areas
|
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
|
|
Relevance
|
SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Goat
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
ab22820 has been purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|
|
Form
|
Liquid
|
|
Concentration
|
0.500 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SART1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for SART1 antibody images, references, products related to ab22820 and other tools.
|
| |
SART1 antibody images:
|
 Western blot - SART1 antibody (ab22820)
SART1 antibody (ab22820) at 0.03 µg/ml + Hela lysate (35µg protein in RIPA buffer)
Predicted band size : 90 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
|
| |
| |
|
Search PubMed (MEDLINE) for references to SART1
|
| |
SART1 antibody - more information
|
Customer reviews (feedback) regarding SART1 antibody |
Customer FAQs regarding SART1 antibody |
Protocols for SART1 antibody |
Price and availability of products related to SART1 antibody |
| |
SART1 antibody - related products:
|
|
|
| |
SART1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about SART1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
