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Products:Cell Biology >> Cell Cycle >> Cell differentiation
Overview
Product name
Anti-SART1 antibody
See all SART1 products (4) ...
Description
Goat polyclonal to SART1
Specificity
ab22820 recognises both the human SART1(259) and SART1(800) proteins.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide: GSSKKHRGEKEAA, corresponding to N terminal amino acids 2-14 of Human SART1.
GSSKKHRGEK EAA
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab22820 has been purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell differentiation
Cell Biology >> Cell Cycle >> Cell differentiation
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Western blot - SART1 antibody (ab22820)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab22820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/64000ELISA: 1/64000
WB: Use a concentration of 0.03 - 0.1 µg/ml.Predicted molecular weight: 90 kDa.
Target
Relevance
SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
Cellular localization
Nuclear
Alternative names
- Ara 1 antibody
- ARA1 antibody
- HOMS 1 antibody
see all
Database links
- Entrez Gene: 9092 Human
- Entrez Gene: 20227 Mouse
- Entrez Gene: 29678 Rat
- NCBI: NP_005137 Human
- Omim: 605941 Human
- SwissProt: O43290 Human
- SwissProt: Q8K155 Mouse
- SwissProt: Q9R270 Mouse
see all
Anti-SART1 antibody images:
Western blot - SART1 antibody (ab22820)
Anti-SART1 antibody (ab22820) at 0.03 µg/ml + Hela lysate (35µg protein in RIPA buffer)
Predicted band size : 90 kDa
Observed band size : 110 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-SART1 antibody (ab22820)
ab22820 has not yet been referenced specifically in any publications.
Publishing research using ab22820? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"