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Anti-SATB1 antibody (ab49061)

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1 question for ab49061

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Question 1

Wednesday 07-December-2011

My colleague and I order anti-SATB1antibody from your company.  We are confused about the dilution of it（ab49061） on IHC-P. Please reply as soon as possible！Thank you!

ANSWER:

  

Thank you for contacting us.

We added IHC -P to the datasheet based on a reference that used the antibody for this application:

Agrelo Ret al. SATB1 defines the developmental context for gene silencing by Xist in lymphoma and embryonic cells. Dev Cell 16:507-16 (2009). (PubMed: 19386260)

Unfortunately, the paper does not include the protocol or dilution. I recommend testing the antibody at a dilution of 1/100 (10 ug/ml). This is assuming you use a standard secondary antibody directly conjugated to HRP or AP for detection. If you use a detection reagent that amplifies signal, for instance a polymer or avidin/biotin complex (ABC), then you may find that you can obtain a good signal at a lower dilution, for example 1/500, with less background staining.

I hope this is helpful. If you are having difficulty obtaining a good specific stain for SATB1, please reply and include some details of your samples and protocol in your reply.

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