|
|
|
|
Western blot - SATB1 antibody (ab70004)
(enlarge)
|
|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody (ab70004)
(enlarge)
|
|
|
|
|
Product Name
|
SATB1 antibody
|
| |
See all SATB1 antibodies (3)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to SATB1
|
|
Immunogen
|
18 amino acid peptide near the amino terminus of the human SATB1 (GenBank accession no. Q01826).
|
|
Reacts with
(species key)
|
Hu, Ms, Rat
|
|
Tested applications
(see key)
|
IHC-P, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
IHC-P: Use at a concentration of 2.5 µg/ml.
WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 100 and 110 kDa (predicted molecular weight: 85 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
SK-N-SH cell lysate
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Chromatin and Nuclear Signaling >> Transcription >> Co-factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
|
|
Relevance
|
Human special AT-rich sequence-binding protein-1 (SATB1) is a matrix protein which binds specifically to the nuclear matrix/scaffold-associating DNAs (MARs/SARs), control elements maintaining independent realms of gene. SATB1 selectively binds double-stranded, special AT-rich DNA sequences in which 1 strand exclusively consists of well-mixed A, T, and C nucleotides (ATC sequences). It complexes mainly with p300 and this complex can bind to the gp91phox promoter and repress transcriptional activity. Its ability to repress transcription is regulated by nuclear matrix binding. It is expressed predominantly in the thymus.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SATB1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for SATB1 antibody images, references, products related to ab70004 and other tools.
|
| |
SATB1 antibody images:
|
 Western blot - SATB1 antibody (ab70004)
Lane 1 : SATB1 antibody (ab70004) at 1 µg/ml
Lane 2 : SATB1 antibody (ab70004) at 2 µg/ml
Lane 1 : SK-N-SH cell lysate
Lane 2 : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 85 kDa
Additional bands at : 100 kDa (possible isoform),110 kDa (possible isoform).
|
| |
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB1 antibody (ab70004)
ab70004 at 2.5µg/ml staining SATB1 in human brain tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
|
| |
| |
|
Search PubMed (MEDLINE) for references to SATB1
|
| |
SATB1 antibody - more information
|
Customer reviews (feedback) regarding SATB1 antibody |
Customer FAQs regarding SATB1 antibody |
Protocols for SATB1 antibody |
Price and availability of products related to SATB1 antibody |
| |
SATB1 antibody - related products:
|
|
|
| |
SATB1 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about SATB1 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
