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Recombinant fragment C-terminal. (Human)
Our Abpromise guarantee covers the use of ab51502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration.|
|WB||1/100. Predicted molecular weight: 81 kDa.|
|IHC-Fr||1/50 - 1/400.|
|IHC-P||1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC||Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab51502 staining cultured rat cortical neuron cells by ICC/IF. Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C. The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400. In the image, red is SATB2 and green is F-actin
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