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Recombinant fragment C-terminal. (Human)
Our Abpromise guarantee covers the use of ab51502 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab51502 staining cultured rat cortical neuron cells by ICC/IF. Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C. The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400. In the image, red is SATB2 and green is F-actin
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