Overview

  • Product nameAnti-SATB2 antibody [SATBA4B10]See all SATB2 primary antibodies ...
  • Description
    Mouse monoclonal [SATBA4B10] to SATB2
  • Tested applicationsIP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Zebrafish
  • Immunogen

    Recombinant fragment C-terminal. (Human)

  • Positive control
    • HT-1080 whole cell lysate. RIPA lysateof HeLacells. HeLa cells. NIH3T3 whole cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, 8mM Dibasic monohydrogen sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Monobasic dihydrogen potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesConcentrated and dialyzed culture medium (5% FBS medium without bovine IgG(<95%approximately)) of hybridoma Purified by ProteinG Filtered through a 0.22 µm membrane and packed aseptically.
  • Clonality Monoclonal
  • Clone numberSATBA4B10
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab51502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100.
ICC/IF 1/5.
IHC-FoFr Use at an assay dependent dilution.
WB 1/100. Predicted molecular weight: 81 kDa.
IHC-Fr 1/50 - 1/400.
IHC-P 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionBinds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • Tissue specificityHigh expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • Involvement in diseaseNote=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • Sequence similaritiesBelongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • Cellular localizationNucleus matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Anti-SATB2 antibody [SATBA4B10] images

  • Anti-SATB2 antibody [SATBA4B10] (ab51502) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Anti Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 81 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation using ab51502 at 1:100 dilution. Sample :NIH3T3 whole cell lysate 25µg. Secondary used at 1:2500.
  • ab51502 staining SATB2 in HeLa cells by Immunocytochemistry 1:200.
  • ab51502 staining cultured rat cortical neuron cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C.  The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.  In the image, red is SATB2 and green is F-actin

    See Abreview

  • ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

    See Abreview

  • ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counter-stained with DAPI (blue).

    See Abreview

  • ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% Donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
    Green: CTIP2 (ab18465) staining
    Blue: TBR1 (ab31940) staining

    See Abreview

References for Anti-SATB2 antibody [SATBA4B10] (ab51502)

This product has been referenced in:
  • Magnani D  et al. Gli3 controls corpus callosum formation by positioning midline guideposts during telencephalic patterning. Cereb Cortex 24:186-98 (2014). Read more (PubMed: 23042737) »
  • Naveau E  et al. Alteration of rat fetal cerebral cortex development after prenatal exposure to polychlorinated biphenyls. PLoS One 9:e91903 (2014). IHC (PFA fixed) ; Rat . Read more (PubMed: 24642964) »

See all 21 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step NGS as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton-X 0.5%
Fixative Paraformaldehyde
Username

Marcos Romualdo Costa

Verified customer

Submitted Jul 18 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Antigen retrieval step None
Sample Rat Tissue sections (E9.5 rat placenta)
Specification E9.5 rat placenta
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 11 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain 50um section)
Specification Brain 50um section
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization Yes - 0.3% Triton X-100 in blocking step
Blocking step Serum as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 31 2012

Of the two rabbit monoclonals, ab92446 (SATB2) and ab92307 (SATB1), ab92446 is more likely to be specific for its target, SATB2, than ab92307 is for SATB1.

Click here (or use the following: http://www.abcam.com/index.html?datasheet=92446). <...

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Thank you for sending the catalogue abID's.

The alignments of the immunogens with the other proteins do suggest that these antibodies may not have the specificity you hope for:

The ab92307 immunogen shares 73% identity (11/15) wit...

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Thank you for contacting us. The concentration of ab51502 is 0.1 mg/ml and should be used at a dilution of 1/100 for both IP and WB. Regarding ab92445, this is a tissue culture supernatant antibody so the concentration is not determined. Generally conc...

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Application Immunohistochemistry (Frozen sections)
Sample Zebra finch Tissue sections (embryonal spinal cord)
Specification embryonal spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.1TX100 in PBS
Blocking step Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Robert Storm

Verified customer

Submitted Feb 06 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for bringing this to our attention. After reading your email I was able to perform an alignmet of the immunogen sequence used to create this product against mouse and rat SATB1 and SATB2 proteins. I found that there was62% homolgy between mur...

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Thank you for your email. I am very glad to hear that you obtained the desired results. As for another SATB2 antibody that is suitable for IP/WB, we have also ab92446: http://www.abcam.com/SATB2-antibody-EPNCIR130A-ab92446.html It was tested in I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"