The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC: 1/200.
IHC-FoFr: Use at an assay dependent dilution (PMID 19619497).
WB: 1/500. Detects a band of approximately 61-65 kDa (predicted molecular weight: 64 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSAX (saxophone) protein is a receptor for dpp. In Drosophila, the saxophone gene encodes a type I receptor that mediates signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta-type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Saxophone gene is expressed ubiquitously. The Saxophone gene gives two products Brk43E and Berk25 and both gene products interact with TGF super family peptide ligands Dpp. Mutations that completely abolish Saxophone activity cause phenotypes that are similar to partial or complete loss of activity of the dpp ligand. The saxophone products have serine threonine kinase activity responsible for phosphorylation and activation of ligands including pMAD, Screw (Scw), and short gastrulation protein (Sog).
Cellular localizationMembrane; single-pass type I membrane protein