AbcamBiochemicals

SB 203580 hydrochloride (ab120235)

Overview

  • Product nameSB 203580 hydrochloride
  • Description
    p38 MAP kinase inhibitor; water soluble
  • Biological descriptionWater soluble, cell permeable p38 MAP kinase inhibitor. Shows selectivity over many other kinases but also shown to have some inhibitory activity against GAK, CK1 , RIP2 c-Raf and GSK3.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120235 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    SB 203580 hydrochloride images

    • MDA-MB-231 cells cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor in MDA-MB-231 cells cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    References for SB 203580 hydrochloride (ab120235)

    This product has been referenced in:
    • Cuenda A & Rousseau S p38 MAP-kinases pathway regulation, function and role in human diseases. Biochim Biophys Acta 1773:1358-75 (2007). Read more (PubMed: 17481747) »
    • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
    • Kumar S  et al. Pyridinylimidazole compound SB 203580 inhibits the activity but not the activation of p38 mitogen-activated protein kinase. Biochem Biophys Res Commun 263:825-31 (1999). Read more (PubMed: 10512765) »

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