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Western blot - SCD antibody [CD.E10] (ab19862)
![Western blot - SCD antibody [CD.E10] (ab19862)](/ps/datasheet/images/ab19862_1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SCD antibody [CD.E10](ab19862)
](\ps\datasheet\images\19\ab19862\SCD-Primary-antibodies-ab19862-1.jpg)
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Product Name
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SCD antibody [CD.E10]
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See all SCD antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [CD.E10] to SCD
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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ELISA, IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 4 µg/ml.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/1000 (See PMID 18697866). Predicted molecular weight: 41.5 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein (probable).
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
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Relevance
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Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to a fat free, high carbohydrate diet. SCD1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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CD.E10
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Isotype
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IgG2b
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SCD antibody [CD.E10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SCD antibody [CD.E10] images, references, products related to ab19862 and other tools.
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SCD antibody [CD.E10] images:
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 Western blot - SCD antibody [CD.E10] (ab19862)
Predicted band size : 41.5 kDa
Western blot using ab19862 on 293 cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SCD antibody [CD.E10](ab19862)
ab19862 staining SCD in human skin.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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References for SCD antibody [CD.E10] (ab19862)
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This product has been used in: (two most recent references)
Hellemans KH et al. Susceptibility of pancreatic beta cells to fatty acids is regulated by LXR/PPARalpha-dependent stearoyl-coenzyme A desaturase. PLoS One 4:e7266 (2009). WB; Rat. PubMed: 19787047
Peter A et al. Individual stearoyl-coa desaturase 1 expression modulates endoplasmic reticulum stress and inflammation in human myotubes and is associated with skeletal muscle lipid storage and insulin sensitivity in vivo. Diabetes 58:1757-65 (2009). PubMed: 19478146
See all 4 publication references for this product.
If you publish research using ab19862 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to SCD
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SCD antibody [CD.E10] - more information
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SCD antibody [CD.E10] - related products:
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SCD antibody [CD.E10] - other tools:
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Contact Abcam with a Technical Enquiry about SCD antibody [CD.E10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - SCD antibody [CD.E10] (ab19862)](/index.html?datasheet=19862#ab19862_1.jpg)
](/index.html?datasheet=19862#SCD-Primary-antibodies-ab19862-1.jpg)
