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Products:Signal Transduction >> Metabolism >> Lipid metabolism
Overview
Product name
Anti-SCD antibody
See all SCD products (4) ...
Description
Rabbit polyclonal to SCD
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCD.
(Peptide available as ab40137.)
Positive control
This antibody gave a positive signal in HEK293 (Human embryonic kidney cell line) and HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: Use a concentration of 2 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 41.5 kDa).
Target
Function
Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
Sequence similarities
Belongs to the fatty acid desaturase family.
Domain
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localization
Endoplasmic reticulum membrane.
Target information above from: UniProt accessionO00767
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ACOD_HUMAN antibody
- Acyl-CoA desaturase antibody
- Delta(9)-desaturase antibody
see all
Database links
- Entrez Gene: 6319 Human
- Entrez Gene: 20249 Mouse
- Entrez Gene: 396670 Pig
- Omim: 604031 Human
- SwissProt: O00767 Human
- SwissProt: Q6RWA7 Pig
- Unigene: 368641 Human
- Unigene: 558396 Human
see all
Anti-SCD antibody images:
Western blot - SCD antibody (ab39969)
All lanes : Anti-SCD antibody (ab39969) at 2 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 15 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 41.5 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-SCD antibody (ab39969)
ICC/IF image of ab39969 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39969, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-SCD antibody (ab39969)
ab39969 has not yet been referenced specifically in any publications.
Publishing research using ab39969? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"