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Western blot - SCD antibody (ab39969)
(enlarge)
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Product Name
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SCD antibody
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See all SCD antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SCD
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SCD. (Peptide available as ab40137.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Pig
(Due to sequence homology)
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 41.5 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in HEK293 (Human embryonic kidney cell line) and HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysates.
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Cellular localization
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Endoplasmic reticulum membrane; Multi-pass membrane protein (probable).
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Lipids / Lipoproteins >> Fatty Acids >> Metabolism
Signal Transduction >> Metabolism >> Lipid metabolism
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Relevance
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Stearoyl-CoA desaturase (SCD) is a regulatory enzyme in lipogenesis. The SCD1 gene is tightly regulated by signals such as insulin, leptin, carbohydrate, fatty acids and temperature. The enzyme is constitutively expressed in adipose tissue and is induced in liver in response to a fat free, high carbohydrate diet. SCD1 expression is higher in female than male mouse. The repression of RNA levels and enzyme activity of hepatic SCD1 by Leptin has been shown to significantly reduce the triglyceride storage, VLDL production and therefore an increase in fatty acid oxidation. Inhibition of SCD1 could be of benefit for treatment of obesity, hepatic steatosis and other metabolic disorders.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.40 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SCD antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SCD antibody images, references, products related to ab39969 and other tools.
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SCD antibody images:
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 Western blot - SCD antibody (ab39969)
All lanes : SCD antibody (ab39969) at 2 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 15 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 41.5 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to SCD
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SCD antibody - more information
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Customer reviews (feedback) regarding SCD antibody |
Customer FAQs regarding SCD antibody |
Protocols for SCD antibody |
Price and availability of products related to SCD antibody |
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SCD antibody - related products:
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SCD antibody - other tools:
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Contact Abcam with a Technical Enquiry about SCD antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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