The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Crucial in the assembly, expression, and functional modulation of the heterotrimeric complex of the sodium channel. The subunit beta-1 can modulate multiple alpha subunit isoforms from brain, skeletal muscle, and heart. Its association with neurofascin may target the sodium channels to the nodes of Ranvier of developing axons and retain these channels at the nodes in mature myelinated axons.
Abundantly expressed in skeletal muscle, heart and brain.
Involvement in disease
Defects in SCN1B are the cause of generalized epilepsy with febrile seizures plus type 1 (GEFS+1) [MIM:604233]. Generalized epilepsy with febrile seizures-plus refers to a rare autosomal dominant, familial condition with incomplete penetrance and large intrafamilial variability. Patients display febrile seizures persisting sometimes beyond the age of 6 years and/or a variety of afebrile seizure types. GEFS+ is a disease combining febrile seizures, generalized seizures often precipitated by fever at age 6 years or more, and partial seizures, with a variable degree of severity. Defects in SCN1B are the cause of Brugada syndrome type 5 (BRS5) [MIM:612838]. A tachyarrhythmia characterized by right bundle branch block and ST segment elevation on an electrocardiogram (ECG). It can cause the ventricles to beat so fast that the blood is prevented from circulating efficiently in the body. When this situation occurs (called ventricular fibrillation), the individual will faint and may die in a few minutes if the heart is not reset.
Belongs to the sodium channel auxiliary subunit SCN1B (TC 8.A.17) family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Sodium channel voltage gated type I beta subunit antibody
Western blot - SCN1B antibody (ab86931)
All lanes : Anti-SCN1B antibody (ab86931) at 1 µg/ml
Lane 1 : Rat brain tissue lysate Lane 2 : Rat testicular tissue lysate Lane 3 : MCF7 whole cell lysate Lane 4 : HeLa whole cell lysate Lane 5 : MM231 whole cell lysate Lane 6 : HT1080 whole cell lysate
Predicted band size : 25 kDa Observed band size : 25 kDa