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Western blot - SCO2 antibody (ab58814)
(enlarge)
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Product Name
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SCO2 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SCO2
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Immunogen
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A 19 amino acid synthetic peptide from near the carboxy terminus of human SCO2.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 30 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human liver tissue lysate
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Cellular localization
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Mitochondrial
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Research areas
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Signal Transduction >> Metabolism >> Vitamins / Minerals
Signal Transduction >> Metabolism >> Mitochondrial
Tags & Cell Markers >> Subcellular Markers >> Organelles >> Mitochondria
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Relevance
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SCO2 (Synthesis of cytochrome c oxidase 2) was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, results in a drop in SCO2 expression which leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in SCO2 are the cause of fatal infantile cardioencephalomyopathy with cytochrome c oxidase deficiency, a disease characterized by hypertrophic cardiomyopathy, lactic acidosis, and gliosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SCO2 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SCO2 antibody images, references, products related to ab58814 and other tools.
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SCO2 antibody images:
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 Western blot - SCO2 antibody (ab58814)
Lane 1 : SCO2 antibody (ab58814) at 0.5 µg/ml
Lane 2 : SCO2 antibody (ab58814) at 1 µg/ml
Lane 3 : SCO2 antibody (ab58814) at 2 µg/ml
Lane 1 : human liver tissue lysate
Lane 2 : human liver tissue lysate
Lane 3 : human liver tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 30 kDa
Observed band size : ~50 kDa (why is the actual band size different from the predicted?)
The difference in size is thought to be a modification in the protein.
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Search PubMed (MEDLINE) for references to SCO2
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SCO2 antibody - more information
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Customer reviews (feedback) regarding SCO2 antibody |
Customer FAQs regarding SCO2 antibody |
Protocols for SCO2 antibody |
Price and availability of products related to SCO2 antibody |
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SCO2 antibody - related products:
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SCO2 antibody - other tools:
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Contact Abcam with a Technical Enquiry about SCO2 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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