Overview
- Product nameAnti-SCP3 antibodySee all SCP3 primary antibodies ...
- DescriptionRabbit polyclonal to SCP3
- Tested applicationsIHC-P, ICC/IF, IHC-Fr, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Rat, Human, Pig
- Immunogen
Synthetic peptide (Human) (C terminal).
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: Tris citrate/phosphate. pH 7-8 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab15093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/400. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: 1/500. |
| IHC-FoFr | IHC-FoFr: 1/500. (see Abreview) |
Target
- FunctionComponent of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
- Tissue specificityTestis-specific.
- Involvement in diseaseDefects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
- Sequence similaritiesBelongs to the XLR/SYCP3 family.
- Cellular localizationNucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
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Database links
- Entrez Gene: 50511 Human
- Entrez Gene: 50511 Human
- Entrez Gene: 20962 Mouse
- Entrez Gene: 25561 Rat
- Omim: 604759 Human
- SwissProt: Q8IZU3 Human
- SwissProt: Q8IZU3 Human
- SwissProt: P70281 Mouse
- SwissProt: Q63520 Rat
- Unigene: 506504 Human
- Unigene: 297977 Mouse
- Unigene: 34889 Rat
see all
Target information above from: UniProt accession
Q8IZU3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- choline phosphotransferase 1 antibodychpt1 antibodyCOR 1 antibody
- COR1 antibodyMGC71888 antibodyRNASCP3 antibodySCP 3 antibodySCP-3 antibodySycp 3 antibodySycp3 antibodySYCP3_HUMAN antibodySynaptonemal complex protein 3 antibody
see all
Anti-SCP3 antibody images
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- SCP3 antibody (ab15093)SCP3 labeled in mouse pachytene preparation (red), using ab15093. CDK2 staining, near teleomeres, is also present (green). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody (ab15093)Ab15093 staining human normal testis tissue. Staining is localized to nuclear compartment.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Immunocytochemistry/ Immunofluorescence - SCP3 antibody (ab15093)This image is a courtesy of Anonymous Abreviewab15093 staining SCP3 in pig spermatocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with Triton x100 and blocking with 0.15% BSA was performed for 30 minutes at 250C. Samples were incubated with primary antibody (1/100: in PBT: PBS + 0.15% BSA+0.1% Tween 20) for 12 hours at 25°C. An Alexa Fluor®594-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/100 as secondary antibody.
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Immunohistochemistry (Frozen sections) - SCP3 antibody (ab15093)This image is courtesy of an anonymous Abreviewab15093 staining SCP3 (green) in Rat testis (spermatocytes) tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/500 in PBS) for 2 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were stained by DAPI (blue).
References for Anti-SCP3 antibody (ab15093)
This product has been referenced in:
- Childs AJ et al. LIN28 Is Selectively Expressed by Primordial and Pre-Meiotic Germ Cells in the Human Fetal Ovary. Stem Cells Dev : (2012). IHC-P ; Human . Read more (PubMed: 22296229) »
- Berkowitz KM et al. Disruption of CHTF18 causes defective meiotic recombination in male mice. PLoS Genet 8:e1002996 (2012). ICC/IF ; Mouse . Read more (PubMed: 23133398) »
