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SDF1 alpha protein (Active) (ab73461)

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Overview

  • Product nameSDF1 alpha protein (Active)See all SDF1 alpha proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 22-90 of Human SDF1 alpha; a non-glycosylated, polypeptide chain containing 68 amino acids and having a molecular mass of 8 kDa. SwissProt P48061.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesThis protein was purified by proprietary chromatographic techniques. Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • FormLyophilised:Reconstitute in sterile 18MOhm/cm water to not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
  • Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    No additives
  • Concentration information loading...
  • Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Sequence notesThe sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73461 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies
  • Application notesBiological Activity: Activity was determined by the ability of ab73461 to chemoattract human peripheral T cells activated with PHA and IL2, using a concentration of 20-80 ng/ml ab73461 .


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C-X-C motif chemokine 12Chemokine (C X C motif) ligand 12CXCL12
        hIRHhSDF-1Intercrine reduced in hepatomasIRHPBSFPre B cell growth stimulating factorPre-B cell growth-stimulating factorSCYB12SDF 1SDF 1 alphaSDF 1aSDF-1SDF-1-alpha(3-67)SDF1SDF1_HUMANSDF1aSDF1BStromal cell derived factor 1TLSFTLSFaTLSFbTPAR1
      see all
  • FunctionChemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P48061 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for SDF1 alpha protein (Active) (ab73461)

    ab73461 has not yet been referenced specifically in any publications.

    Publishing research using ab73461 ? Please let us know so that we can cite the reference in this datasheet

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    What protocol was used to test this protein in a chemo-attraction assay?

    Thank you very much for your call last week and for your patience while I've been in touch with my colleagues regarding your enquiry.
    The SDF1 alpha protein, Ab73461, was tested in an activity assay byan outside laboratory, and unfortunately we do no...

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