Overview
Product nameSDF1 alpha protein (Active)See all SDF1 alpha proteins and peptides ...
Protein descriptionRecombinant full length Rat SDF1 alpha; 68 amino acids, 7.9 kDa. Swiss ID = Q9QZD1
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab79959 is greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per µg (1EU/µg).
Biological activityBiological Activity :
Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50.0-100.0 ng/ml.
FormLyophilised:Centrifuge the vial prior to opening. Reconstitute in water
to a concentration of 0.1-1.0 mg/ml. Do not vortex. This
solution can be stored at 2-8°C; for up to 1 week. For
extended storage, it is recommended to further dilute in a
buffer containing a carrier protein (example 0.1% BSA)
and store in working aliquots at -20°C; to -80°C;. Working aliquots stored with a carrier protein are stable for at least 3 months at
-20°C; to -80°C;.
Avoid repeated freeze/thaw cycles.
Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Concentration information loading...
Sequence notesKPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab79959
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
-
Alternative names
C-X-C motif chemokine 12Chemokine (C X C motif) ligand 12Chemokine (CXC motif) ligand 12
Chemokine C X C motif ligand 12Chemokine ligand 12CXCL 12CXCL12hIRHhSDF-1Intercrine reduced in hepatomasIRHPBSFPre B cell growth stimulating factorPre-B cell growth-stimulating factorSCYB 12SCYB12SDF 1 alphaSDF 1ASDF 1BSDF-1SDF-1-alpha(3-67)SDF1SDF1_HUMANSDF1aSDF1BStromal cell derived factor 1TLSFTLSF aTLSF bTLSFaTLSFbTPAR 1TPAR1
see all
FunctionChemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
Post-translational
modificationsProcessed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
Cellular localizationSecreted.
References for SDF1 alpha protein (Active) (ab79959)
ab79959
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
