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Overview

  • Product nameSDF1 alpha proteinSee all SDF1 alpha proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Mouse) 7.9kDa
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notes>99% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg (1EU/µg).
  • FormLyophilised:We recommend a quick spin followed by reconstitution in sterile distilled water to a concentration of 0.1-1.0 mg/ml. (The protein may appear as a haze or film, which is difficult to see at the bottom of the vial.)
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 10mM Acetic acid
    Endotoxin level is <0.1 ng per µg (1EU/µg).
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab51939 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesBiological Activity: Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 50-100 ng/ml.
    SDS-PAGE: Use at an assay dependent dilution.
    HPLC: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        C-X-C motif chemokine 12cxcl12hIRH
        hSDF-1Intercrine reduced in hepatomasIRHPBSFPre-B cell growth-stimulating factorSCYB12SDF 1 alphaSDF-1SDF-1-alpha(3-67)SDF1SDF1_HUMANSDF1aSDF1BStromal cell derived factor 1TLSFTPAR1
      see all
  • FunctionChemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the Lyn kinase. Stimulates migration of monocytes through its receptor, CXCR4, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through Lyn kinase.
  • Sequence similaritiesBelongs to the intercrine alpha (chemokine CxC) family.
  • Post-translational
    modifications    Processed forms SDF-1-beta(3-72) and SDF-1-alpha(3-67) are produced after secretion by proteolytic cleavage of isoforms Beta and Alpha, respectively. The N-terminal processing is probably achieved by DPP4. Isoform Alpha is first cleaved at the C-terminus to yield a SDF-1-alpha(1-67) intermediate before being processsed at the N-terminus. The C-terminal processing of isoform Alpha is reduced by binding to heparin and, probably, cell surface proteoglycans.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P48061 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for SDF1 alpha protein (ab51939)

    ab51939 has not yet been referenced specifically in any publications.

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