Overview
Product nameSDF1 beta protein (Active)See all SDF1 beta proteins and peptides ...
Protein descriptionRecombinant full length SDF1 beta protein (Mouse). This is a non-glycosylated, polypeptide chain containing 72 amino acids and has a MW of 8513 Dalton. Swiss ID = P40224.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesThis product is purified by proprietary chromatographic techniques.
Purity: Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
FormLyophilised:It is recommended to reconstitute the lyophilized product with 18MO-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Concentration information loading...
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Sequence notesThe sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab73464
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| Functional Studies |
|
| SDS-PAGE |
|
Application notesBiological Activity: Use at an assay dependent dilution. The specific activity as determined by its ability to chemo-attract human monocytes at 50-100 ng/ml.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
-
Alternative names
Chemokine (C X C motif) ligand 12Chemokine (CXC motif) ligand 12CXCL 12
CXCL12hIRHPBSFPre B cell growth stimulating factorSCYB 12SCYB12SDF 1 betaSDF 1aSDF1SDF1aSDF1bStromal cell derived factor 1TLSFTLSF aTLSFaTPAR 1TPAR1
see all
RelevanceStromal cell derived factor beta 1 (SDF1) is a 70 amino acid CXC chemokine originally cloned from a bone marrow stromal cell line. This member of the intercrine alpha (chemokine CxC) family is a chemoattractant active on T lymphocytes, monocytes, but not neutrophils. Targeted deletion of SDF1 gene resulted in defects of B cell lymphopoiesis and bone marrow myelopoiesis in mice. SDF1 has been shown to be chemotactic for lymphocytes. In addition, SDF1 was recently reported to be a ligand for CXCR4 (LESTR/fusin) and a co receptor for HIV1 entry into T cells (SDF1 binding to CXCR4 inhibits HIV1 entry).
Cellular localizationSecreted
References for SDF1 beta protein (Active) (ab73464)
ab73464
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
