Anti-SDF4 antibody (ab113413)
Key features and details
- Goat polyclonal to SDF4
- Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SDF4 antibody
See all SDF4 primary antibodies -
Description
Goat polyclonal to SDF4 -
Host species
Goat -
Specificity
ab113413 is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2). -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Goat, Cow, Dog -
Immunogen
Synthetic peptide:
EYKVKFLASKGHSEK
by a Cysteine residue linker, corresponding to internal sequence amino acids 161-175 of Human SDF4 (NP_057631.1; NP_057260.2). -
Positive control
- WB: U251, HeLa and Jurkat cell lysate. Flow Cyt: A431 cells ICC/IF: U2OS cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab11343 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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Flow Cyt
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment.
Isoform 5 may be involved in the exocytosis of zymogens by pancreatic acini. -
Tissue specificity
Ubiquitous. Isoform 5 is expressed in pancreas. -
Sequence similarities
Belongs to the CREC family.
Contains 6 EF-hand domains. -
Cellular localization
Golgi apparatus lumen and Cytoplasm. Cell projection > bleb. Isoform 5 colocalizes with STX3 and STXBP1 isoform 2 at the plasma membrane and cell surface blebs. - Information by UniProt
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Database links
- Entrez Gene: 528783 Cow
- Entrez Gene: 51150 Human
- Entrez Gene: 20318 Mouse
- Entrez Gene: 155173 Rat
- Omim: 614282 Human
- SwissProt: Q3ZBZ1 Cow
- SwissProt: Q9BRK5 Human
- SwissProt: Q61112 Mouse
see all -
Alternative names
- 45 kDa calcium-binding protein antibody
- Cab45 antibody
- CAB45_HUMAN antibody
see all
Images
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All lanes : Anti-SDF4 antibody (ab113413) at 0.1 µg/ml
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U251 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDa -
Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab113413 has not yet been referenced specifically in any publications.