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ab50672 has been referenced in 1 publications.
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Human HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 µg/mL Anti-Sec23 antibody. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Predicted band size : 86 kDa
HeLa cell homogenates were fractionated on a discontinuous 10–30% OptiPrepTM gradient. Marker proteins indicate mitochondria (complex II), MAM (calnexin), rER (Sec61α), transitional ER (Sec23)(ab50672), pan-ER (PDI), endosomes (MPR46), and cytosol (GAPDH). Fractions are assigned their predominant content.
Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584
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