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Immunocytochemistry/ Immunofluorescence - SEC23 antibody (ab50672)
Human HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 µg/mL Anti-Sec23 antibody. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Western blot - SEC23 antibody (ab50672)
Predicted band size : 86 kDa
HeLa cell homogenates were fractionated on a discontinuous 10–30% OptiPrepTM gradient. Marker proteins indicate mitochondria (complex II), MAM (calnexin), rER (Sec61α), transitional ER (Sec23)(ab50672), pan-ER (PDI), endosomes (MPR46), and cytosol (GAPDH). Fractions are assigned their predominant content.
Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584
SEC23 antibody for Western blot in Human (50672)
SEC23 antibody for Western blot in Mouse (50672)
SEC23 antibody for Immunocytochemistry in Human (50672)
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