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Anti-SEC23 antibody (ab50672)

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Overview

Product name

Anti-SEC23 antibody
See all SEC23 products (4) ...

Description

Rabbit polyclonal to SEC23

Tested applications

ICC, WB, ICC/IFmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Predicted to work with

Cow, Xenopus laevis, Fruit fly (Drosophila melanogaster), Zebrafish, Orangutan

Immunogen

Synthetic peptide corresponding to amino acid residues 1-18 of human Sec23A with C-terminal added cysteine conjugated to KLH. The corresponding sequence is identical in Rat and Mouse Sec23A and differs by one amino acid in Human and Mouse Sec23B.

MTTYLEFIQQ NEERDGVR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human HeLa cells for immunofluorescence and whole cell extracts of Mouse NIH-3T3 and Rat ROS cells for WB.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Clonality

Polyclonal

Isotype

IgG

  • Immunocytochemistry/ Immunofluorescence - SEC23 antibody (ab50672)Immunocytochemistry/ Immunofluorescence - SEC23 antibody (ab50672) image (enlarge)

  • Western blot - SEC23 antibody (ab50672)Western blot - SEC23 antibody (ab50672) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab50672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at a concentration of 5 - 10 µg/ml (recommended using Human HeLa cells).

WB: Use at a concentration of 1 - 2 µg/ml. (recommended using whole cell extracts of mouse NIH-3T3 and rat ROS cells, applying a chemiluminescent detection reagent). Detects a band of approximately 75 kDa (predicted molecular weight: 86 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.

Involvement in disease

Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.

Sequence similarities

Belongs to the SEC23/SEC24 family. SEC23 subfamily.

Cellular localization

Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.

Target information above from: UniProt accessionQ15436 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Protein transport protein Sec23A antibody
  • SC23A_HUMAN antibody
  • SEC23-related protein A antibody
  • sec23a antibody

Database links

Anti-SEC23 antibody images:

  Immunocytochemistry/ Immunofluorescence - SEC23 antibody (ab50672)

Immunocytochemistry/ Immunofluorescence - SEC23 antibody (ab50672)

Human HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 µg/mL Anti-Sec23 antibody. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.

  Western blot - SEC23 antibody (ab50672)

Western blot - SEC23 antibody (ab50672)



Predicted band size : 86 kDa


HeLa cell homogenates were fractionated on a discontinuous 10–30% OptiPrepTM gradient. Marker proteins indicate mitochondria (complex II), MAM (calnexin), rER (Sec61α), transitional ER (Sec23)(ab50672), pan-ER (PDI), endosomes (MPR46), and cytosol (GAPDH). Fractions are assigned their predominant content.

Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584

References for Anti-SEC23 antibody (ab50672)

This product has been referenced in:

  • Bui Met al. Rab32 modulates apoptosis onset and mitochondria-associated membrane (MAM) properties. J Biol Chem 285:31590-602 (2010). WB; Human.Read more (PubMed: 20670942) »

See 1 publication for this product

Publishing research using ab50672? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"