Anti-SENP3 antibody [EP4360] (ab124790)
Key features and details
- Rabbit monoclonal [EP4360] to SENP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-SENP3 antibody [EP4360]
See all SENP3 primary antibodies -
Description
Rabbit monoclonal [EP4360] to SENP3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide within Human SENP3 aa 550-650 (C terminal). The exact sequence is proprietary.
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Positive control
- MCF-7, Jurkat and HeLa cell lysates.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP4360 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124790 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/1000 - 1/10000. Predicted molecular weight: 65 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 65 kDa. |
Target
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Function
Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. -
Sequence similarities
Belongs to the peptidase C48 family. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (By similarity). Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress. - Information by UniProt
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Database links
- Entrez Gene: 26168 Human
- Entrez Gene: 80886 Mouse
- Entrez Gene: 303245 Rat
- Omim: 612844 Human
- SwissProt: Q9H4L4 Human
- SwissProt: Q9EP97 Mouse
- Unigene: 513926 Human
- Unigene: 733104 Human
see all -
Alternative names
- SENP3 antibody
- SENP3_HUMAN antibody
- Sentrin / SUMO specific protease 3 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab124790 has been referenced in 3 publications.
- Han Y et al. SENP3-mediated TIP60 deSUMOylation is required for DNA-PKcs activity and DNA damage repair. MedComm (2020) 3:e123 (2022). PubMed: 35356800
- Wang P et al. Inhibition of SUMO2/3 antagonizes isoflurane-induced cancer-promoting effect in hepatocellular carcinoma Hep3B cells. Oncol Lett 21:274 (2021). PubMed: 33732350
- Gao SS et al. TIP60 K430 SUMOylation attenuates its interaction with DNA-PKcs in S-phase cells: Facilitating homologous recombination and emerging target for cancer therapy. Sci Adv 6:eaba7822 (2020). PubMed: 32832608