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Overview

  • Product nameSEPT5 protein (Tagged-His Tag)See all SEPT5 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 14-369 (356 amino acids) of Human SEPT5 with N terminal His tag. Predicted mwt: 42 kDa; observed mwt: 47 kDa; UniProt ID = Q99719.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution with 53 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notes(Amino acid sequence (Sequence determined by 5' Sequencing))
    PEDKQDIDKQYVGFATLPNQVHRKSV KKGFDFTLMVAGESGLGKSTLVHSLFL TDLYKDRKLLS AEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVN NT ECWKPITDYVDQQFEQYFRDESGLNRKNIQDNRVHC CLYFISPFGHGLRPVDV GFMKALHEKVNIVPLIAKADC LVPSEIRKLKERIREEIDKFGIHVYQFPECDS DEDEDF KQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVE VENQAHC DFVKLRNMLIRTHMHDLKDVTCDVHYENYRA HCIQQMTSKLTQDSRMESPIPI LPLPTPDAETEKLIRM KDEELRRMQEMLQRMKQQMQDQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91710 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        5 SepCDCRELCDCrel 1
        CDCrel-1CDCREL1Cell division control related protein 1Cell division control-related protein 1H5HCDCREL 1OTTHUMP00000197271OTTHUMP00000197272OTTHUMP00000197273OTTHUMP00000197274OTTHUMP00000197275OTTHUMP00000197276OTTHUMP00000197396OTTHUMP00000197410Peanut like 1Peanut like 1 homologPeanut like protein 1Peanut-like protein 1Platelet glycoprotein Ib beta chainPNUTL1SEPT5SEPT5_HUMANSeptin 5Septin-5
      see all
  • FunctionFilament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion.
  • Tissue specificityExpressed at high levels in the CNS, as well as in heart and platelets (at protein level).
  • Sequence similaritiesBelongs to the septin family.
  • Post-translational
    modifications    In platelets, phosphorylated in response to thrombin, phorbol-12-myristate-13-acetate and collagen.
  • Cellular localizationCytoplasm. Cytoplasm > cytoskeleton. In platelets, found in areas surrounding alpha-granules.

    • Target information above from: UniProt accession Q99719 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SEPT5 protein (Tagged-His Tag) images

    • SDS-PAGE - SEPT5 protein (Tagged-His Tag) (ab91710)
      SDS-PAGE - SEPT5 protein (Tagged-His Tag) (ab91710)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SEPT5 protein (Tagged-His Tag) (ab91710)

    ab91710 has not yet been referenced specifically in any publications.

    Publishing research using ab91710 ? Please let us know so that we can cite the reference in this datasheet

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