You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-SERCA3 ATPase antibodySee all SERCA3 ATPase primary antibodies ...
  • Description
    Rabbit polyclonal to SERCA3 ATPase
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog
  • Immunogen

    Synthetic peptide within residues: LISGWLFFRY LAIGVYVGLA TVAAATWWFV YDAEGPHINF YQLRNFLKCS , corresponding to internal sequence amino acids 828-877 of Human SERCA3 ATPase (NP_005164).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlJurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab85753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 114 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionThis magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
  • Tissue specificityFound in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues.
  • Sequence similaritiesBelongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
  • Cellular localizationNucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.

    • Target information above from: UniProt accession Q93084 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Adenosine triphosphatase calcium antibodyAT2A3_HUMAN antibodyATP2A3 antibody
        ATPase Ca(2+) transporting ubiquitous antibodyATPase Ca++ transporting ubiquitous antibodyCalcium pump 3 antibodyCalcium translocating P type ATPase antibodySarco/endoplasmic reticulum Ca2+ ATPase antibodySarcoplasmic/endoplasmic reticulum calcium ATPase 3 antibodySERCA 3 antibodySERCA3 antibodySERCA3b antibodySR Ca(2+) ATPase 3 antibodySR Ca(2+)-ATPase 3 antibody
      see all

    Anti-SERCA3 ATPase antibody images

    • Western blot - SERCA3 ATPase antibody (ab85753)
      Western blot - SERCA3 ATPase antibody (ab85753)
      Anti-SERCA3 ATPase antibody (ab85753) at 1 µg/ml (5% skim milk / PBS buffer) + Jurkat cell lysate at 10 µg

      Secondary
      anti-Rabbit IgG HRP at 1/50000 dilution

      Predicted band size : 114 kDa
      Observed band size : 109 kDa (why is the actual band size different from the predicted?)

    References for Anti-SERCA3 ATPase antibody (ab85753)

    ab85753 has not yet been referenced specifically in any publications.

    Publishing research using ab85753 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab85753 Submit a Question for ab85753

    There are currently no Abreviews or Questions for ab85753.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"