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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab47731? Please let us know so that we can cite the reference in this datasheet
ab47731 has been referenced in 1 publications.
- Tseng MY et al. Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncol 45:771-6 (2009). WB, IHC-P; Human. PubMed: 19213596
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SERPINB1 antibody (ab47731)
All lanes : Anti-SERPINB1 antibody (ab47731) at 1/1000 dilution
Lane 1 : MH-S cell lysate
Lane 2 : MH-S cell lysate
Lane 3 : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 43 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa (possible degradation product).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SERPINB1 antibody(ab47731)
ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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