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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-SERPINB1 antibody
See all SERPINB1 products (5) ...
Description
Rabbit polyclonal to SERPINB1
Specificity
This antibody is specific for SERPINB1.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide based on the aminoterminal end of mature human SERPINB1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> SERPINs
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000.Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IHC-P: Use at an assay dependent dilution.
Target
Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3.
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily.
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP30740
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Database links
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
Anti-SERPINB1 antibody images:
Western blot - SERPINB1 antibody (ab47731)
All lanes : Anti-SERPINB1 antibody (ab47731) at 1/1000 dilution
Lane 1 : MH-S cell lysate
Lane 2 : MH-S cell lysate
Lane 3 : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size : 43 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa (possible degradation product).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-SERPINB1 antibody(ab47731)
ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Protocols
References for Anti-SERPINB1 antibody (ab47731)
This product has been referenced in:
- Tseng MYet al. Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncol 45:771-6 (2009). WB, IHC-P; Human.Read more (PubMed: 19213596) »
See 1 publication for this product
Publishing research using ab47731? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"