Western blot - KMT7 / SETD7/ SET7/9 antibody [s4E5] (ab14820)

Predicted band size : 50 kDa


Western blot analysis of recombinant SET7 using ab14820 at 1/1000. Proteins were visualised using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system.

Immunofluorescence - SET7 /SET9 antibody [s4E5] (ab14820)

Immunoflouresence was carried out using mouse cells in culture. Conditions: 4% PFA fixation and dilution with 0.5% triton, 10mg/ml BSA in PBS. Dilution of ab14820 used: 1/50. Green = ab14820 detected with secondary antibody (Alexa 488). Blue = DAPI stain.

Katia Ancelin

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - KMT7 / SETD7/ SET7/9 antibody [s4E5] (ab14820)

ab14820 at 1/25 dilution staining KMT7 / SETD7 / SET7/9 in mouse pancrease tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 5% serum for 1 hour at 20ºC and then incubated with ab14820 for 18 hours at 4ºC. A Cy2 conjugated donkey polyclonal to mouse Ig, diluted 1/20, was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Flow Cytometry-Anti-SETD7 antibody [s4E5] - ChIP Grade(ab14820)

Overlay histogram showing HeLa cells stained with ab14820 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14820, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.