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Product name
Anti-SETMAR antibody
See all SETMAR products (4) ...
Description
Goat polyclonal to SETMAR
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: RWQKCVDCNGSYFD, corresponding to C terminal amino acids 658-671 of Human SETMAR.
RWQKCVDCNGSYFD
Positive control
Daudi lysate.
General notes
Principal Names – SETMAR; SET domain and mariner transposase fusion gene.
Official Gene Symbol - SETMAR.
GenBank Accession Number – NP_006506.
LocusLink ID - 6419 (human).
Gene Ontology terms - transposase activity
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - SETMAR antibody (ab3823)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Western Blot – Approx 70 - 75 kDa band seen in Daudi lysate.
Recommended for use at 1.0 - 3.0µg/ml.
Not tested in other applications.
Optimal working dilutions / concentrations should be determined by the end user.
Target
Function
Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA.
Tissue specificity
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
Sequence similarities
In the N-terminal section; belongs to the histone-lysine methyltransferase family.
In the C-terminal section; belongs to the mariner transposase family.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain.
Domain
The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue.
Cellular localization
Nucleus. Chromosome.
Target information above from: UniProt accessionQ53H47
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Histone lysine N methyltransferase antibody
- Histone lysine N methyltransferase SETMAR antibody
- Hsmar 1 antibody
see all
Database links
- Entrez Gene: 6419 Human
- Omim: 609834 Human
- SwissProt: Q53H47 Human
- Unigene: 716054 Human
Anti-SETMAR antibody images:
Western blot - SETMAR antibody (ab3823)
ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.
References for Anti-SETMAR antibody (ab3823)
This product has been referenced in:
- Van Duyne R et al. Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR. Retrovirology 5:40 (2008). WB; Human.Read more (PubMed: 18498648) »
See 1 publication for this product
Publishing research using ab3823? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"