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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Anti-SFMBT1 antibody
See all SFMBT1 products (4) ...
Mouse polyclonal to SFMBT1
ICC/IF, WBmore details
Reacts with
Human
Full length protein, corresponding to amino acids 1-866 of Human SFMBT1
SFMBT1 transfected 293T cell lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein G purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Our Abpromise guarantee covers the use of ab68199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 10 µg/ml
WB: 1/500 - 1/1000.Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
Expressed in all cell lines and normal tissues tested, including the thymus.
Contains 4 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain.
Target information above from: UniProt accessionQ9UHJ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - SFMBT1 antibody (ab68199)

All lanes : Anti-SFMBT1 antibody (ab68199) at 1/500 dilution
Lane 1 : SFMBT1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 98 kDa
Observed band size : 98 kDa
Immunocytochemistry/ Immunofluorescence - SFMBT1 antibody (ab68199)

Immunofluorescence using ab68199 on HeLa cell [antibody concentration 10 ug/ml].
ab68199 has not yet been referenced specifically in any publications.
Publishing research using ab68199? Please let us know so that we can cite the reference in this datasheet
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All lanes : Anti-SFMBT1 antibody (ab68199) at 1/500 dilution
Lane 1 : SFMBT1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 98 kDa
Observed band size : 98 kDa

Immunofluorescence using ab68199 on HeLa cell [antibody concentration 10 ug/ml].
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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