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Anti-SFMBT1 antibody (ab77419) at 1 µg/ml + Recombinant tagged human SFMBT1 fragment at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 98 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 38 kDa.
All lanes : Anti-SFMBT1 antibody (ab77419) at 1 µg/ml
Lane 1 : SFMBT1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 98 kDa
Observed band size : 98 kDa
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