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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Overview
Product name
Anti-SFMBT1 antibody
See all SFMBT1 products (4) ...
Description
Mouse monoclonal to SFMBT1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 121-230 of Human SFMBT1 (NP_057413) with tag.
Positive control
SFMBT1 transfected 293T cell lysate; Recombinant tagged human SFMBT1 fragment.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
ELISA: Use at an assay dependent dilution.
Target
Tissue specificity
Expressed in all cell lines and normal tissues tested, including the thymus.
Sequence similarities
Contains 4 MBT repeats.
Contains 1 SAM (sterile alpha motif) domain.
Target information above from: UniProt accessionQ9UHJ3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp434L243 antibody
- MGC93349 antibody
- OTTMUSP00000030378 antibody
see all
Database links
- Entrez Gene: 51460 Human
- Omim: 607319 Human
- SwissProt: Q9UHJ3 Human
- Unigene: 343679 Human
Anti-SFMBT1 antibody images:
Western blot - SFMBT1 antibody (ab77419)
Anti-SFMBT1 antibody (ab77419) at 1 µg/ml + Recombinant tagged human SFMBT1 fragment at 0.2 µg
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 98 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 38 kDa.
Western blot - SFMBT1 antibody (ab77419)
All lanes : Anti-SFMBT1 antibody (ab77419) at 1 µg/ml
Lane 1 : SFMBT1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 98 kDa
Observed band size : 98 kDa
References for Anti-SFMBT1 antibody (ab77419)
ab77419 has not yet been referenced specifically in any publications.
Publishing research using ab77419? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"