Anti-SRSF3 antibody (ab73891)
Key features and details
- Rabbit polyclonal to SRSF3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SRSF3 antibody
See all SRSF3 primary antibodies -
Description
Rabbit polyclonal to SRSF3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SRSF3 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 19 kDa.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 19 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
May be involved in RNA processing in relation with cellular proliferation and/or maturation. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6428 Human
- Omim: 603364 Human
- SwissProt: P84103 Human
- Unigene: 405144 Human
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Alternative names
- arginine/serine-rich 3 antibody
- Pre mRNA splicing factor SRP20 antibody
- Pre-mRNA-splicing factor SRP20 antibody
see all
Images
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All lanes : Anti-SRSF3 antibody (ab73891) at 1/500 dilution
Lane 1 : extracts from HeLa cells
Lane 2 : extracts from Jurkat cells
Lane 3 : extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 19 kDa
Observed band size: 19 kDa -
Left panel: immunofluorescence analysis of MCF7 cells, using 1/500-1/1000 ab73891; Right panel: same, after pre-incubation with the immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab73891 has been referenced in 9 publications.
- Dennison BJC et al. Srsf3 mediates alternative RNA splicing downstream of PDGFRα signaling in the facial mesenchyme. Development 148:N/A (2021). PubMed: 34184034
- Zhou Y et al. Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in Leukemia. Cancer Discov 10:1388-1409 (2020). PubMed: 32444465
- Fuentes-Fayos AC et al. Splicing machinery dysregulation drives glioblastoma development/aggressiveness: oncogenic role of SRSF3. Brain 143:3273-3293 (2020). PubMed: 33141183
- Kumar D et al. Degradation of splicing factor SRSF3 contributes to progressive liver disease. J Clin Invest 129:4477-4491 (2019). PubMed: 31393851
- Roundtree IA et al. YTHDC1 mediates nuclear export of N6-methyladenosine methylated mRNAs. Elife 6:N/A (2017). PubMed: 28984244
- Jiang P et al. Fyn/heterogeneous nuclear ribonucleoprotein E1 signaling regulates pancreatic cancer metastasis by affecting the alternative splicing of integrin ß1. Int J Oncol 51:169-183 (2017). WB . PubMed: 28560430
- Zheng X et al. TRA2ß controls Mypt1 exon 24 splicing in the developmental maturation of mouse mesenteric artery smooth muscle. Am J Physiol Cell Physiol 308:C289-96 (2015). PubMed: 25428883
- Wang Q et al. Homer1 alternative splicing is regulated by gonadotropin-releasing hormone and modulates gonadotropin gene expression. Mol Cell Biol 34:1747-56 (2014). PubMed: 24591653
- Sen S et al. Splicing factor SRSF3 is crucial for hepatocyte differentiation and metabolic function. Nat Commun 4:1336 (2013). WB ; Mouse . PubMed: 23299886