Anti-SGK1 antibody [Y238] (ab32374)

Overview

• Product nameAnti-SGK1 antibody [Y238]See all SGK1 primary antibodies ...
• Description
  Rabbit monoclonal [Y238] to SGK1
• Tested applicationsWB, IHC-P, Flow Cyt, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues of human SGK1.

• Positive controlWB: A431 and NIH3T3 cell lysates. IHC-P: Human kidney carcinoma.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• Concentration information loading...
• Clonality Monoclonal
• Clone numberY238
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionProtein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
• Tissue specificityExpressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 protein kinase domain.
• DomainIsoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
• Post-translational
  modificationsRegulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
  Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
• Cellular localizationCell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.

Target information above from: UniProt accession O00141 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6446 Human
  • Entrez Gene: 20393 Mouse
  • Entrez Gene: 29517 Rat
  • Omim: 602958 Human
  • SwissProt: O00141 Human
  • SwissProt: Q9WVC6 Mouse
  • SwissProt: Q06226 Rat
  • Unigene: 510078 Human

  • Unigene: 28405 Mouse
  • Unigene: 4636 Rat

  see all

• Alternative names
  Serine/threonine protein kinase SGK antibodySerine/threonine protein kinase Sgk1 antibodySerine/threonine-protein kinase Sgk1 antibody

  Serum and glucocorticoid regulated kinase antibodySerum/glucocorticoid regulated kinase 1 antibodySerum/glucocorticoid regulated kinase 1 antibodySerum/glucocorticoid regulated kinase antibodySerum/glucocorticoid-regulated kinase 1 antibodySGK 1 antibodySGK antibodySGK1 antibodySGK1_HUMAN antibody

  see all

Anti-SGK1 antibody [Y238] images

• 

  Western blot - SGK1 antibody [Y238] (ab32374)
  All lanes : Anti-SGK1 antibody [Y238] (ab32374) at 1/500 dilution
  
  Lane 1 : A431 cell lysate
  Lane 2 : NIH3T3 cell lysate
  
  
  Predicted band size : 49 kDa
  Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - SGK1 antibody [Y238] (ab32374)
  Ab32374, at a 1/50 dilution, staining SGK1 in paraffin embedded human kidney carcinoma tissue sections by Immunohistochemistry.
• 

  Flow Cytometry-Anti-SGK1 antibody [Y238](ab32374)
  Overlay histogram showing HEK293 cells stained with ab32374 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32374, 1/100) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.