Overview
- Product nameAnti-SGK1 antibodySee all SGK1 primary antibodies ...
- DescriptionRabbit polyclonal to SGK1
- Tested applicationsWB, ICC/IF, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SGK1.
(Peptide available as ab43605.)
- Positive controlab43606 gave a positive signal in the following Whole Cell Lysates: Hela, Jurkat, A431, HEK293 and NIH 3T3. IF/ICC: HeLa cell line.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab43606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa).Can be blocked with SGK1 peptide (ab43605). |
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
| IHC-P | IHC-P: 1/2000. |
Target
- FunctionProtein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
- Tissue specificityExpressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. - DomainIsoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
- Post-translational
modificationsRegulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid. - Cellular localizationCell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
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Database links
- Entrez Gene: 6446 Human
- Entrez Gene: 20393 Mouse
- Entrez Gene: 29517 Rat
- Omim: 602958 Human
- SwissProt: O00141 Human
- SwissProt: Q9WVC6 Mouse
- SwissProt: Q06226 Rat
- Unigene: 510078 Human
- Unigene: 28405 Mouse
- Unigene: 4636 Rat
see all
Target information above from: UniProt accession
O00141
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Serine/threonine protein kinase SGK antibodySerine/threonine protein kinase Sgk1 antibodySerine/threonine-protein kinase Sgk1 antibody
- Serum and glucocorticoid regulated kinase antibodySerum/glucocorticoid regulated kinase 1 antibodySerum/glucocorticoid regulated kinase 1 antibodySerum/glucocorticoid regulated kinase antibodySerum/glucocorticoid-regulated kinase 1 antibodySGK 1 antibodySGK antibodySGK1 antibodySGK1_HUMAN antibody
see all
Anti-SGK1 antibody images
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Immunocytochemistry/ Immunofluorescence - Anti-SGK1 antibody (ab43606)ICC/IF image of ab43606 stained HeLa cells. The cells were 4% formaldeyhde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43606, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - SGK1 antibody (ab43606)All lanes : Anti-SGK1 antibody (ab43606) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
SGK1 contains Glu/Lys-rich regions and a number of phosphorylated Ser/Thr sites (SwissProt data) which may explain why the observed band is running higher than the predicted molecular weight. -
Western blot - SGK1 antibody (ab43606)All lanes : Anti-SGK1 antibody (ab43606) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withSGK1 peptide (ab43605) at 1 µg/ml SGK1 contains Glu/Lys-rich regions and a number of phosphorylated Ser/Thr sites (SwissProt data) which may explain why the observed band is running higher than the predicted molecular weight.
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate withSGK1 peptide (ab43605) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 68 kDa (why is the actual band size different from the predicted?)
References for Anti-SGK1 antibody (ab43606)
This product has been referenced in:
- Spangle JM & Münger K The human papillomavirus type 16 E6 oncoprotein activates mTORC1 signaling and increases protein synthesis. J Virol 84:9398-407 (2010). WB ; Human . Read more (PubMed: 20631133) »
