Overview

  • Product nameAnti-SGK1 antibodySee all SGK1 primary antibodies ...
  • Description
    Rabbit polyclonal to SGK1
  • Tested applicationsWB, ICC/IF, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SGK1.

    (Peptide available as ab43605.)

  • Positive control
    • ab43606 gave a positive signal in the following Whole Cell Lysates: Hela, Jurkat, A431, HEK293 and NIH 3T3. IF/ICC: HeLa cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab43606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 49 kDa).Can be blocked with Human SGK1 peptide (ab43605).
ICC/IF Use a concentration of 10 µg/ml.
IHC-P 1/2000.

Target

  • FunctionProtein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
  • Tissue specificityExpressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • DomainIsoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
  • Post-translational
    modifications
    Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
    Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
  • Cellular localizationCell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
  • Target information above from: UniProt accession O00141 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • OTTHUMP00000017247 antibody
    • Serine/threonine protein kinase SGK antibody
    • Serine/threonine protein kinase Sgk1 antibody
    • Serine/threonine-protein kinase Sgk1 antibody
    • Serum and glucocorticoid regulated kinase antibody
    • Serum/glucocorticoid regulated kinase 1 antibody
    • Serum/glucocorticoid regulated kinase 1 antibody
    • Serum/glucocorticoid regulated kinase antibody
    • Serum/glucocorticoid-regulated kinase 1 antibody
    • SGK 1 antibody
    • SGK antibody
    • SGK1 antibody
    • Sgk1 variant i3 antibody
    • SGK1_HUMAN antibody
    see all

Anti-SGK1 antibody images

  • ICC/IF image of ab43606 stained HeLa cells. The cells were 4% formaldeyhde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab43606, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-SGK1 antibody (ab43606) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    SGK1 contains Glu/Lys-rich regions and a number of phosphorylated Ser/Thr sites (SwissProt data) which may explain why the observed band is running higher than the predicted molecular weight.
  • All lanes : Anti-SGK1 antibody (ab43606) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SGK1 peptide (ab43605) at 1 µg/ml
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SGK1 peptide (ab43605) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    SGK1 contains Glu/Lys-rich regions and a number of phosphorylated Ser/Thr sites (SwissProt data) which may explain why the observed band is running higher than the predicted molecular weight.

References for Anti-SGK1 antibody (ab43606)

This product has been referenced in:
  • Spangle JM & Münger K The human papillomavirus type 16 E6 oncoprotein activates mTORC1 signaling and increases protein synthesis. J Virol 84:9398-407 (2010). WB ; Human . Read more (PubMed: 20631133) »

See 1 Publication for this product

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The good news first: both antibodies are suitable for your purpose. However, the ab59337 has actually been used with mouse stratium. Please see the attached reference.

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The lab let me know that they haven't tested the antibodyto determine cross-reactivity with sgk2 and 3 but they blasted the immunogenagainst theHuman sequences and it only picked up sgk1.

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab43606 with the order number 998916. To check the status of the orde...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Specification kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6
Permeabilization Yes - 1% TBST
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 24 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Intestinal adenoma)
Specification Intestinal adenoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Mr. Antibody Solutions

Verified customer

Submitted Jun 22 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon Cancer)
Specification Colon Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
Permeabilization No
Blocking step 5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jun 10 2009

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