Anti-SGK1 antibody (ab55678)

Overview

• Product nameAnti-SGK1 antibodySee all SGK1 primary antibodies ...
• Description
  Mouse monoclonal to SGK1
• Tested applicationsWB, ICC/IF, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant full length protein, corresponding to amino acids 1-432 of Human SGK1

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  PBS, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • Other Kinases

Applications

Target

• FunctionProtein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
• Tissue specificityExpressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
• Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
  Contains 1 AGC-kinase C-terminal domain.
  Contains 1 protein kinase domain.
• DomainIsoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
• Post-translational
  modificationsRegulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
  Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
• Cellular localizationCell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.

Target information above from: UniProt accession O00141 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6446 Human
  • Omim: 602958 Human
  • SwissProt: O00141 Human
  • Unigene: 510078 Human

• Alternative names
  Serine/threonine protein kinase SGK antibodySerine/threonine protein kinase Sgk1 antibodySerine/threonine-protein kinase Sgk1 antibody

  Serum and glucocorticoid regulated kinase antibodySerum/glucocorticoid regulated kinase 1 antibodySerum/glucocorticoid regulated kinase antibodySerum/glucocorticoid-regulated kinase 1 antibodySGK 1 antibodySGK antibodySGK1 antibodySGK1_HUMAN antibody

  see all

Anti-SGK1 antibody images

• 

  Immunofluorescence - SGK1 antibody (ab55678)
  SGK1 antibody (ab55678) used in immunofluorescence at 10ug/ml on HeLa cells.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGK1 antibody (ab55678)
  SGK1 antibody (ab55678) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.