Overview

  • Product nameAnti-SGK1 antibodySee all SGK1 primary antibodies ...
  • Description
    Rabbit polyclonal to SGK1
  • Tested applicationsICC/IF, ELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human SGK1 around the phosphorylation site of serine 422

  • Positive control
    • Human lung carcinoma tissue. Jurkat cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab63527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
ELISA
WB
IHC-P
  • Application notesELISA: 1/5000.
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionProtein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, KCNA3/Kv1.3 or EAAT1. Isoform 2 exhibited a greater effect on cell plasma membrane expression of ENaC and Na(+) transport than isoform 1.
    • Tissue specificityExpressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung. Isoform 2 is strongly expressed in brain and pancreas, weaker in heart, placenta, lung, liver and skeletal muscle.
    • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 protein kinase domain.
    • DomainIsoform 2 subcellular localization at the plasma membrane is mediated by the sequences within the first 120 amino acids.
    • Post-translational
      modifications
      Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3-kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1.
      Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells. Isoform 2 shows enhanced stability. Isoform 2 resistance to proteasomal degradation is mediated by the sequences within the first 120-amino acid.
    • Cellular localizationCell membrane and Cytoplasm. Nucleus. Endoplasmic reticulum. Nuclear, upon phosphorylation.
    • Target information above from: UniProt accession O00141 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • OTTHUMP00000017247 antibody
      • Serine/threonine protein kinase SGK antibody
      • Serine/threonine protein kinase Sgk1 antibody
      • Serine/threonine-protein kinase Sgk1 antibody
      • Serum and glucocorticoid regulated kinase antibody
      • Serum/glucocorticoid regulated kinase 1 antibody
      • Serum/glucocorticoid regulated kinase antibody
      • Serum/glucocorticoid-regulated kinase 1 antibody
      • SGK 1 antibody
      • SGK antibody
      • SGK1 antibody
      • Sgk1 variant i3 antibody
      • SGK1_HUMAN antibody
      see all

    Anti-SGK1 antibody images

    • ab63527, at 1/50 - 1/100 dilution, staining SGK1 in human lung carcinoma tissue by immunohistochemistry using paraffin-embedded tissue, in the absence (left) and presence (right) of immunising peptide.
    • All lanes : Anti-SGK1 antibody (ab63527) at 1/500 dilution

      Lane 1 : Jurkat cell extract
      Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.


      Predicted band size : 49 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab63527 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63527, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-SGK1 antibody (ab63527)

    ab63527 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us with the problem. I have looked through the information provided and have the following questions and suggestions for the customer. Please let me know the answers of the customer. As usual, if the antibody vial is faulty, we...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"