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Product name
Anti-SGLT1 antibody
See all SGLT1 products (7) ...
Description
Rabbit polyclonal to SGLT1
Tested applications
IHC-P, WB, Dot Blot, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Sheep, Human
Immunogen
Synthetic peptide, corresponding to amino acids 603-623 of Human SGLT1
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGLT1 antibody (ab14685)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab14685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 55-62 kDa.
Dot Blot
Dot: 1/4000.Dot: 1/4000.
ELISA
ELISA: 1/4000.ELISA: 1/4000.
Target
Function
Actively transports glucose into cells by Na(+) cotransport with a Na(+) to glucose coupling ratio of 2:1. Efficient substrate transport in mammalian kidney is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules.
Tissue specificity
Expressed mainly in intestine and kidney.
Involvement in disease
Defects in SLC5A1 are the cause of congenital glucose/galactose malabsorption (GGM) [MIM:606824]. GGM is an intestinal monosaccharide transporter deficiency. It is an autosomal recessive disorder manifesting itself within the first weeks of life. It is characterized by severe diarrhea and dehydration which are usually fatal unless glucose and galactose are eliminated from the diet.
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Post-translational
modifications
N-glycosylation is not necessary for the cotransporter function.
Cellular localization
Membrane.
Target information above from: UniProt accessionP13866
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D22S675 antibody
- High affinity sodium glucose cotransporter 1 antibody
- High affinity sodium glucose cotransporter antibody
see all
Database links
- Entrez Gene: 6523 Human
- Entrez Gene: 20537 Mouse
- Entrez Gene: 25552 Rat
- Omim: 182380 Human
- SwissProt: P13866 Human
- SwissProt: Q8C3K6 Mouse
- SwissProt: P53790 Rat
- SwissProt: P53791 Sheep
see all
Anti-SGLT1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SGLT1 antibody (ab14685)
ab14685 (2µg/ml) staining SGLT1 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the apical epithelium cells of the villi.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-SGLT1 antibody (ab14685)
This product has been referenced in:
- Leiprecht N et al. Regulation of Na(+)-coupled glucose carrier SGLT1 by human papillomavirus 18 E6 protein. Biochem Biophys Res Commun 404:695-700 (2011). WB; Human.Read more (PubMed: 21156162) »
- Chou CW et al. HDAC Inhibition Decreases the Expression of EGFR in Colorectal Cancer Cells. PLoS One 6:e18087 (2011). WB; Human.Read more (PubMed: 21464950) »
See all 6 publications for this product
Publishing research using ab14685? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"