Anti-SGSH/HSS antibody (ab72617)
Key features and details
- Mouse polyclonal to SGSH/HSS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SGSH/HSS antibody
See all SGSH/HSS primary antibodies -
Description
Mouse polyclonal to SGSH/HSS -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SGSH/HSS. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: NP_000190.1 -
Positive control
- SGSH/HSS transfected 293T cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: PBS
1X PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 57 kDa.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 57 kDa. |
Target
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Involvement in disease
Defects in SGSH are the cause of mucopolysaccharidosis type 3A (MPS3A) [MIM:252900]; also known as Sanfilippo syndrome A. MPS3A is a severe form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. MPS3A is characterized by earlier onset, rapid progression of symptoms and shorter survival. -
Sequence similarities
Belongs to the sulfatase family. -
Post-translational
modificationsThe conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 6448 Human
- Omim: 605270 Human
- SwissProt: P51688 Human
- Unigene: 31074 Human
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Alternative names
- Heparan sulfate sulfatase antibody
- Heparan sulphate sulphatase antibody
- HSS antibody
see all
Images
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All lanes : Anti-SGSH/HSS antibody (ab72617) at 1/500 dilution
Lane 1 : SGSH/HSS transfected 293T cell line
Lane 2 : Non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab72617 has been referenced in 1 publication.
- Magat J et al. Intracerebroventricular dosing of N-sulfoglucosamine sulfohydrolase in mucopolysaccharidosis IIIA mice reduces markers of brain lysosomal dysfunction. J Biol Chem 298:102625 (2022). PubMed: 36306823