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- Secondary antibodies
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Product name
Anti-SGTA antibody - Azide free
See all SGTA products (4) ...
Description
Chicken polyclonal to SGTA - Azide free
Specificity
This antibody is specific for SGTA.
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
Synthetic peptide, corresponding to amino acids 61-313 of SGTA, near C-terminus.
Epitope
C-terminal
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgY
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Protein Trafficking >> Chaperones >> Heat Shock Proteins
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Western blot - SGTA antibody - Azide free (ab37706)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab37706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF: 1/100.
Target
Function
Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the SGT family.
Contains 3 TPR repeats.
Domain
The second tetratricopeptide repeat (TPR 2) mediates the interaction with SARS-CoV accessory protein 7a.
Target information above from: UniProt accessionO43765
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha SGT antibody
- Alpha-SGT antibody
- AlphaSGT antibody
see all
Database links
- Entrez Gene: 6449 Human
- Entrez Gene: 52551 Mouse
- Omim: 603419 Human
- SwissProt: Q6FIA9 Human
- SwissProt: O43765 Human
- SwissProt: Q3TN35 Mouse
- SwissProt: Q8BJU0 Mouse
- Unigene: 203910 Human
see all
Anti-SGTA antibody - Azide free images:
Western blot - SGTA antibody - Azide free (ab37706)
Anti-SGTA antibody - Azide free (ab37706) at 1/2000 dilution + E. coli-derived fusion protein at 0.5 µg
Secondary
Goat anti-IgY-HRP. at 1/1000 dilution
Predicted band size : 34 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?)
A fusion protein with 6xhis-NusA, which adds 470 amino acids (around 55-60 kDa to the protein) was used in this western blot. The predicted SGTA protein is around 34 kDa. Therefore, the higher than expected band size was obtained.
References for Anti-SGTA antibody - Azide free (ab37706)
This product has been referenced in:
- Andreyeva A et al. CHL1 is a selective organizer of the presynaptic machinery chaperoning the SNARE complex. PLoS One 5:e12018 (2010). WB; Mouse.Read more (PubMed: 20711454) »
See 1 publication for this product
Publishing research using ab37706? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"