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Products:Cardiovascular >> Blood >> Other

Anti-SH2D1A/SAP antibody (ab50422)

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Overview

Product name

Anti-SH2D1A/SAP antibody
See all SH2D1A/SAP products (4) ...

Description

Goat polyclonal to SH2D1A/SAP

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Pig, Macaque Monkey

Immunogen

Synthetic peptide: C-QYPVEKKSSARSTQ, corresponding to internal sequence amino acids 99-112 of Human SH2D1A/SAP

C-QYPVEKKS SARSTQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human Lymph Node lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality

Polyclonal

Isotype

IgG

Research areas

Immunology >> Immune System Diseases >> Antiviral Signaling
Cardiovascular >> Blood >> Other

  • Western blot - SH2D1A/SAP antibody (ab50422)Western blot - SH2D1A/SAP antibody (ab50422) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab50422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.2 - 0.6 µg/ml.Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).

Target

Relevance

The cytoplasmic protein encoded by the SH2D1A gene plays an essential role in controlling EBV infection. It is expressed in T and NK cells, but not in B cells or in granulocytes. SH2D1A is expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Defects in SH2D1A are a cause of X-linked lymphoproliferative disease (XLPD) also known as Duncan disease. XLPD is characterized by an immunodeficiency following Epstein-Barr virus (EBV) infection. Symptoms include fatal mononucleosis, hypogammaglobulinemia, pancytopenia or malignant lymphoma.

Cellular localization

Cytoplasmic

Alternative names

  • SH2 domain protein 1A antibody
  • DSHP antibody
  • Duncan disease SH2 protein antibody
  • SAP antibody
  • Signaling lymphocyte activation molecule associated protein antibody
  • SLAM associated protein antibody
  • T cell signal transduction molecule SAP antibody
see all

Database links

Anti-SH2D1A/SAP antibody images:

  Western blot - SH2D1A/SAP antibody (ab50422)

Western blot - SH2D1A/SAP antibody (ab50422)

Anti-SH2D1A/SAP antibody (ab50422) at 0.2 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).

Predicted band size : 14 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)


Primary incubation was 1 hour. Detected by chemiluminescence.

References for Anti-SH2D1A/SAP antibody (ab50422)

ab50422 has not yet been referenced specifically in any publications.

Publishing research using ab50422? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"