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Western blot - SH2D1A/SAP antibody (ab50422)
(enlarge)
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Product Name
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SH2D1A/SAP antibody
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See all SH2D1A/SAP antibodies (2)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to SH2D1A/SAP
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Immunogen
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Synthetic peptide: C-QYPVEKKSSARSTQ, corresponding to internal sequence amino acids 99-112 of Human SH2D1A/SAP
BLAST 'C-QYPVEKKSSARSTQ' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Mcq, Pig
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:32,000.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
Some minor background is detected and is blocked by the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Lymph Node lysate.
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Cellular localization
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Cytoplasmic
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Research areas
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Immunology >> Immune System Diseases >> Antiviral Signaling
Cardiovascular >> Blood >> Other
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Relevance
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The cytoplasmic protein encoded by the SH2D1A gene plays an essential role in controlling EBV infection. It is expressed in T and NK cells, but not in B cells or in granulocytes. SH2D1A is expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver.
Defects in SH2D1A are a cause of X-linked lymphoproliferative disease (XLPD)also known as Duncan disease. XLPD is characterized by an immunodeficiency following Epstein-Barr virus (EBV)infection. Symptoms include fatal mononucleosis, hypogammaglobulinemia, pancytopenia or malignant lymphoma.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SH2D1A/SAP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SH2D1A/SAP antibody images, references, products related to ab50422 and other tools.
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SH2D1A/SAP antibody images:
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 Western blot - SH2D1A/SAP antibody (ab50422)
SH2D1A/SAP antibody (ab50422) at 0.2 µg/ml + Human Lymph Node lysate (35µg protein in RIPA buffer).
Predicted band size : 14 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence.
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Search PubMed (MEDLINE) for references to SH2D1A/SAP
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SH2D1A/SAP antibody - more information
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Customer reviews (feedback) regarding SH2D1A/SAP antibody |
Customer FAQs regarding SH2D1A/SAP antibody |
Protocols for SH2D1A/SAP antibody |
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SH2D1A/SAP antibody - related products:
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SH2D1A/SAP antibody - other tools:
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Contact Abcam with a Technical Enquiry about SH2D1A/SAP antibody |
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