Anti-SH3 containing Grb 2 like 1 protein antibody (ab86605)

Overview

  • Product name
    Anti-SH3 containing Grb 2 like 1 protein antibody
    See all SH3 containing Grb 2 like 1 protein primary antibodies
  • Description
    Rabbit polyclonal to SH3 containing Grb 2 like 1 protein
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 225 and 275 of human SH3 containing Grb 2 like 1 protein (NP_003016.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Implicated in endocytosis. May recruit other proteins to membranes with high curvature.
  • Tissue specificity
    Ubiquitous. Higher expression in pancreas, placenta, prostate, testis and uterus.
  • Involvement in disease
    Note=In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene.
  • Sequence similarities
    Belongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • Domain
    An N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
  • Cellular localization
    Cytoplasm. Early endosome membrane. Associated with postsynaptic endosomes in hippocampal neurons.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNSA1 antibody
    • EEN antibody
    • EEN fusion partner of MLL antibody
    • Endophilin-2 antibody
    • Endophilin-A2 antibody
    • Extra 11 19 leukemia fusion gene antibody
    • Extra eleven-nineteen leukemia fusion gene protein antibody
    • Fusion partner of MLL antibody
    • MGC111371 antibody
    • SH3 containing Grb 2 like 1 protein antibody
    • SH3 containing protein EEN antibody
    • SH3 domain GRB2 like 1 antibody
    • SH3 domain protein 2B antibody
    • SH3 domain-containing GRB2-like protein 1 antibody
    • SH3D2B antibody
    • SH3G1_HUMAN antibody
    • Sh3gl1 antibody
    • SH3P8 antibody
    see all

Images

  • All lanes : Anti-SH3 containing Grb 2 like 1 protein antibody (ab86605) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 41 kDa


    Exposure time : 30 seconds
  • Detection of SH3 containing Grb 2 like 1 protein by Western Blot of Immunprecipitate.
    ab86605 at 1µg/ml staining SH3 containing Grb 2 like 1 protein in HeLa whole cell lysate immunoprecipitated using ab86605 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86605 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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