Anti-SHANK1 antibody [N22/21] (ab94576)
Key features and details
- Mouse monoclonal [N22/21] to SHANK1
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SHANK1 antibody [N22/21]
See all SHANK1 primary antibodies -
Description
Mouse monoclonal [N22/21] to SHANK1 -
Host species
Mouse -
Specificity
ab94576 does not react with SHANK2 and SHANK3 -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Rat SHANK1 aa 450-700.
Database link: Q9WV48 -
Positive control
- Rat brain lysate
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General notes
The clone number has been updated from S22-21 to N22/21, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N22/21 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94576 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 225 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 225 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. Plays a role in the structural and functional organization of the dendritic spine and synaptic junction. -
Tissue specificity
Expressed in brain particularly in the amygdala, hippocampus, substantia nigra and thalamus. Isoform 2 seems to be expressed ubiquitously. -
Sequence similarities
Belongs to the SHANK family.
Contains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > synapse. Postsynaptic density of neuronal cells. - Information by UniProt
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Database links
- Entrez Gene: 50944 Human
- Entrez Gene: 243961 Mouse
- Entrez Gene: 78957 Rat
- Omim: 604999 Human
- SwissProt: Q9Y566 Human
- SwissProt: D3YZU1 Mouse
- SwissProt: Q9WV48 Rat
- Unigene: 274255 Human
see all -
Alternative names
- GKAP/SAPAP interacting protein antibody
- OTTHUMP00000174437 antibody
- SH3 and multiple ankyrin repeat domains 1 antibody
see all
Images
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Western blot detection of Shank1 in Rat brain membrane lysates using ab94576 at 1:1000 dilution.
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paraffin-embedded, bouin's-fixed mouse backskin stained for SHANK1 using ab94576 at 1/100 dilution in immunohistochemical analysis. FITC Goat Anti-Mouse (green) at 1/50 dilution was used as the secondary antibody. Filaggrinlike staining (upper layer aggregations of staining) is observed.
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Overlay histogram showing SH-SH5Y cells stained with ab94576 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab94576, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab94576 has been referenced in 3 publications.
- Collins SM & Galvez R Neocortical SHANK1 regulation of forebrain dependent associative learning. Neurobiol Learn Mem 155:173-179 (2018). PubMed: 30053575
- Collins SM et al. SHANK1 is differentially expressed during development in CA1 hippocampal neurons and astrocytes. Dev Neurobiol 78:363-373 (2018). PubMed: 29218848
- He Y et al. Effects of curcumin on synapses in APPswe/PS1dE9 mice. Int J Immunopathol Pharmacol 29:217-25 (2016). PubMed: 26957323