Anti-SHANK2 antibody [N23B/49] (ab94575)
Key features and details
- Mouse monoclonal [N23B/49] to SHANK2
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SHANK2 antibody [N23B/49]
See all SHANK2 primary antibodies -
Description
Mouse monoclonal [N23B/49] to SHANK2 -
Host species
Mouse -
Specificity
Recognizes SHANK1, SHANK2, SHANK3. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Fusion protein corresponding to Rat SHANK2 aa 50-350.
Database link: Q9QX74 -
Positive control
- Rat brain lysate
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General notes
The clone number has been updated from S23B-49 to N23B/49, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N23B/49 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab94575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | ||
ICC/IF |
Notes |
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ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 159 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. -
Tissue specificity
Expressed in epithelial cells (at protein level). All isoforms except isoform 7 are expressed predominantly in brain, with highest levels in olfactory bulb, cerebral cortex, cerebellum, central gray matter and hippocampus. Moderate levels of expression are seen in the caudate putamen, thalamic nuclei and brain stem. In cerebellum primarily expressed in Purkinje cells. Isoform 7 is not expressed in brain but expressed in liver, cholangiocytes and thymus. Isoform 7 is present in pancreas, colonic mucosa and thymocytes (at protein level). -
Sequence similarities
Belongs to the SHANK family.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
Developmental stage
Expressed during early postnatal brain development, especially in the caudate putamen and thalamic nuclei. Expression in the cerebral cortex, the hippocampus and the cerebellum is moderate to high at P5 and shows a stable expression throughout development. Isoforms 1, 2, 4 and 6 are predominantly expressed in cerebellum up to the age of approximately 3 weeks. Isoform 1 expression decreases during development of cortex but slightly increases in cerebellum. -
Domain
The PDZ domain is required for interaction with GRID2, PLCB3, SLC9A3 and CFTR. -
Cellular localization
Apical cell membrane. Cytoplasm. Cell junction, synapse. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, growth cone. Cell projection, dendritic spine. Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Present in the dendritic spines of cerebellar Purkinje cells (By similarity). Colocalizes with cortactin in growth cones in differentiating hippocampal neurons. Colocalized with PDE4D to the apical membrane of colonic crypt cells. - Information by UniProt
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Database links
- Entrez Gene: 22941 Human
- Entrez Gene: 171093 Rat
- Omim: 603290 Human
- SwissProt: Q9UPX8 Human
- SwissProt: Q9QX74 Rat
- Unigene: 268726 Human
- Unigene: 14545 Rat
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Alternative names
- AUTS17 antibody
- Cortactin binding protein 1 antibody
- Cortactin SH3 domain-binding protein antibody
see all
Images
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4% formaldehyde-fixed (for 15 minutes at room temperature) SK-N-BE (Human neuroblastoma cells) labeling SHANK2 using ab94575 at 1/100 dilution (for 60 minutes at room temperature). Goat Anti-Mouse ATTO 488 was used as the secondary at 1/200 dilution for 60 minutes at room temperature. Localization in the sytplasm is observed.
Counterstains: Phalloidin Texas Red F-Actin stain (Red). DAPI nuclear stain (Blue).
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Western blot detection of SHANK2 in Rat braim membrane lysates using ab94575 at 1:1000 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab94575 has been referenced in 1 publication.
- Fan XY et al. Neonatal Sevoflurane Exposure Impairs Learning and Memory by the Hypermethylation of Hippocampal Synaptic Genes. Mol Neurobiol 58:895-904 (2021). PubMed: 33052583