Recombinant Anti-SHANK3 antibody [S69] (ab93607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [S69] to SHANK3
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-SHANK3 antibody [S69]
See all SHANK3 primary antibodies -
Description
Mouse monoclonal [S69] to SHANK3 -
Host species
Mouse -
Specificity
No cross-reactivity with SHANK1 or SHANK2. -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow -
Immunogen
Synthetic peptide corresponding to Rat SHANK3 aa 800-900 (internal sequence).
Database link: Q9JLU4 -
General notes
The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S69 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 186 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 190 kDa (predicted molecular weight: 186 kDa). |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction. -
Tissue specificity
Expressed in the cerebral cortex and the cerebellum. -
Involvement in disease
Note=A chromosomal aberration involving SHANK3 is found in patients with chromosome 22q13.3 deletion syndrome. Translocation t(12;22)(q24.1;q13.3) with APPL2/DIP13B.
Note=Defects in SHANK3 are associated with autism spectrum disorders (ASD). ASD are characterized by impairments in reciprocal social interaction and communication as well as restricted and stereotyped patterns of interest and activities. ASD include forms with moderate to severe cognitive impairment and milder forms with higher cognitive ability (Asperger syndrome).
Defects in SHANK3 are the cause of schizophrenia type 15 (SCZD15) [MIM:613950]. SCZD15 is a complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
Sequence similarities
Contains 6 ANK repeats.
Contains 1 PDZ (DHR) domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH3 domain. -
Cellular localization
Cytoplasm. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Postsynaptic density of neuronal cells. - Information by UniProt
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Database links
- Entrez Gene: 85358 Human
- Entrez Gene: 58234 Mouse
- Entrez Gene: 59312 Rat
- Omim: 606230 Human
- SwissProt: Q9BYB0 Human
- SwissProt: Q4ACU6 Mouse
- SwissProt: Q9JLU4 Rat
- Unigene: 149035 Human
see all -
Alternative names
- AI841104 antibody
- DEL22q13.3 antibody
- KIAA1650 antibody
see all
Images
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Anti-SHANK3 antibody [S69] (ab93607) at 1/1000 dilution + Rat brain membrane lysate at 15 µg
Secondary
Sheep Anti-Mouse IgG-HRP
Predicted band size: 186 kDa -
Immunohistochemical analysis of mouse backskin tissue sections labling SHANK3 with ab93607 at 1/100 dilution. FITC Goat anti-mouse at 1/50 was used as the secondary antibody.
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Overlay histogram showing SH-SY5Y cells stained with ab93607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab93607 has been referenced in 4 publications.
- Chen B et al. SHANK1 facilitates non-small cell lung cancer processes through modulating the ubiquitination of Klotho by interacting with MDM2. Cell Death Dis 13:403 (2022). PubMed: 35468874
- Man W et al. SHANK3 Co-ordinately Regulates Autophagy and Apoptosis in Myocardial Infarction. Front Physiol 11:1082 (2020). PubMed: 32982797
- Gassowska-Dobrowolska M et al. Prenatal Exposure to Valproic Acid Affects Microglia and Synaptic Ultrastructure in a Brain-Region-Specific Manner in Young-Adult Male Rats: Relevance to Autism Spectrum Disorders. Int J Mol Sci 21:N/A (2020). PubMed: 32443651
- Zhang Y et al. Effect of intraperitoneal or intracerebroventricular injection of streptozotocin on learning and memory in mice. Exp Ther Med 16:2375-2380 (2018). PubMed: 30186480