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Overview

  • Product nameSHMT2 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 376-504 (129 amino acids) of Human SHMT2 with an N terminal His tag; Predicted MWt 15 kDa (UniProt P34897).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 125 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesGTDNHLVLVDLRPKGLDGARAERVLELVSITANKNTCPGD RSAITPGGLR LGAPALTSRQFREDDFRRVVDFIDEGVN IGLEVKSKTAKLQDFKSFLLKD SETSQRLANLRQRVEQ FARAFPMPGFDEH
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91960 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        GLY A+GLYAglycine auxotroph A, human complement for hamster
        Glycine hydroxymethyltransferaseGLYM_HUMANmitochondrialSerine hydroxymethyltransferaseSerine hydroxymethyltransferase 2 (mitochondrial)Serine hydroxymethyltransferase mitochondrialSerine methylaseSHMTSHMT 2SHMT2
      see all
  • FunctionContributes to the de novo mitochondrial thymidylate biosynthesis pathway. Required to prevent uracil accumulation in mtDNA. Interconversion of serine and glycine. Associates with mitochondrial DNA.
  • PathwayOne-carbon metabolism; tetrahydrofolate interconversion.
  • Sequence similaritiesBelongs to the SHMT family.
  • Cellular localizationMitochondrion. Mitochondrion matrix > mitochondrion nucleoid. Mitochondrion inner membrane.

    • Target information above from: UniProt accession P34897 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SHMT2 protein (Tagged-His Tag) images

    • SDS-PAGE - SHMT2 protein (Tagged-His Tag) (ab91960)
      SDS-PAGE - SHMT2 protein (Tagged-His Tag) (ab91960)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SHMT2 protein (Tagged-His Tag) (ab91960)

    ab91960 has not yet been referenced specifically in any publications.

    Publishing research using ab91960 ? Please let us know so that we can cite the reference in this datasheet

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