Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Proteins and Peptides
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-SHP1 antibody
See all SHP1 products (19) ...
Description
Rabbit polyclonal to SHP1
Specificity
Recognises the recombinant phosphatase domain fusion protein (60 kDa) in Western blots. Predicted molecular weight for SHP1 is 70 kDa. When used in IHC staining on human tonsil stains the mantle zone of B cell follicles and T cells in the interfollicular area. Seems to be selective for small lymphoid cells, which is in keeping with published data eg. Khoury J et al. and Oka T et al.
Tested applications
IHC-P, WB, ICC/IFmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SHP1.
(Peptide available as ab822 08.)
Positive control
This antibody gave a positive signal in SHP1 Recombinant protein and Jurkat Whole Cell Lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Hematopoietic system development
Cancer >> Signal transduction >> G protein signaling >> GPCR
Cancer >> Cell cycle >> Kinases/phosphatases >> Phosphatases
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Phosphatases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).
ICC/IF: Use a concentration of 1 µg/ml.
Target
Function
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation.
Tissue specificity
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Sequence similarities
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
Post-translational
modifications
Phosphorylated on serine and tyrosine residues.
Cellular localization
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II.
Target information above from: UniProt accessionP29350
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 70 kda SHP1L protein antibody
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
see all
Database links
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Entrez Gene: 116689 Rat
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- SwissProt: P81718 Rat
- Unigene: 63489 Human
see all
Anti-SHP1 antibody images:
- SHP1 antibody (ab2020)
ab2020 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).
Picture of a B cell follicle.
The antibody shows strong staining of mantle zone cells and some staining of a few cells in the light zone of the germinal centre. This is in keeping with published data (see Khoury J et al and Oka T et al.).
Immunocytochemistry/ Immunofluorescence - SHP1 antibody (ab2020)
ICC/IF image of ab2020 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab2020, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - SHP1 antibody (ab2020)
All lanes : Anti-SHP1 antibody (ab2020) at 1 µg/ml
Lane 1 : PTPN6 - Recombinant Protein (Human) at 0.1 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 60 kDa
Observed band size : 68,92 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
ab2020 detects a band of ~90 kDa corresponding to the predicted molecular weight of the recombinant SHP1 protein. In whole cell lysates ab2020 detects a 68 kDa band which we believe to be endogenous SHP1. It also binds to a 45 kDa protein, the identity of which is unknown.
Western blot - SHP1 antibody (ab2020)
All lanes : Anti-SHP1 antibody (ab2020) at 1/2000 dilution
Lane 1 : PTP1B (PTPN1)
Lane 2 : TCPTP (PTPN2)
Lane 3 : PTPH1 (PTPN3)
Lane 4 : MEG1 (PTPN4)
Lane 5 : STEP (PTPN5)
Lane 6 : SHP1 (PTPN6)
Lane 7 : HePTP (PTPN7)
Lane 8 : SHP2 (PTPN11)
Lane 9 : BDP1 (PTPN18)
Predicted band size : 60 kDa
Observed band size : 60 kDa
Recombinant PTP phosphatase domains were probed with ab2020 at 1/2000. The antibody recognises the recombinant SHP1 fusion protein at ~60 kDa.
Data provided by Purely Proteins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SHP1 antibody (ab2020)
ab2020 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).
The antibody shows staining of T cells in the interfollicullar area. This is in keeping with published data (see Khoury J et al and Oka T et al.).
References for Anti-SHP1 antibody (ab2020)
This product has been referenced in:
- Alam HB et al. Alterations in gene expression after induction of profound hypothermia for the treatment of lethal hemorrhage. J Trauma 68:1084-98 (2010). WB; Rat.Read more (PubMed: 20453763) »
- Insabato L et al. Elevated Expression of the Tyrosine Phosphatase SHP-1 Defines a Subset of High-Grade Breast Tumors. Oncology 77:378-384 (2009). WB, IHC-P; Human.Read more (PubMed: 20090381) »
See all 5 publications for this product
Publishing research using ab2020? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"